Recombinant Mouse Anti-Pfs25 Antibody (4B7) (CAT#: FAMAB-0226-CN)

Figure 1 Competition ELISA with mouse monoclonal antibodies against rabbit, monkey, or human anti-Pfs25 serum.

A fixed amount of mouse monoclonal anti-Pfs25 antibody 4B7 was tested with 2-fold dilutions of anti-Pfs25 serum from rabbit, monkey and humans. The amount of Pfs25-specific antibodies in the diluted serum (competitor) is shown on the x-axis and the O.D. value from the individual mouse monoclonal antibody is shown on the y-axis. The mean and standard deviation were calculated from two independent experiments. For some data points, the standard deviations are too small and are not visible.

Cheru, L., Wu, Y., Diouf, A., Moretz, S. E., Muratova, O. V., Song, G., ... & Miura, K. (2010). The IC50 of anti-Pfs25 antibody in membrane-feeding assay varies among species. Vaccine, 28(27), 4423-4429.

Figure 2 Immunoblot of reduced and non-reduced algae-produced Pfs25 with monoclonal transmission blocking antibodies.

Reduced and non-reduced a-Pfs25 was resolved by SDS-PAGE, transferred to nitrocellulose, anddetected with anti-Pfs25 4B7 mAb.

Gregory, J. A., Li, F., Tomosada, L. M., Cox, C. J., Topol, A. B., Vinetz, J. M., & Mayfield, S. (2012). Algae-produced Pfs25 elicits antibodies that inhibit malaria transmission. PloS one, 7(5), e37179.

Figure 3 Western blot analysis of P.falciparum mixed sexual stage lysates with anti-Pfs25-4B7 mAbs.

Reduced and non-reduced sexual stagelysates were resolved by SDS-PAGE and transferred to nitrocellulose. Blots were probed with anti-Pfs25-4B7 mAbs. (R-reduced, N-non-reduced)

Gregory, J. A., Li, F., Tomosada, L. M., Cox, C. J., Topol, A. B., Vinetz, J. M., & Mayfield, S. (2012). Algae-produced Pfs25 elicits antibodies that inhibit malaria transmission. PloS one, 7(5), e37179.

Figure 4 Indirect immunofluorescence using immune sera from mice immunized with algae-produced Pfs25 on in-vitro cultured P. falciparum gametocytes, gametes, and zygotes.

DNA was stained using DAPI (blue) and antibody binding was visualized using Alexa Fluor 488-conjugated rabbit anti-mouse IgG (green) for anti-Pfs25 4B7 mAbs. Scale bars, 1 μm.

Gregory, J. A., Li, F., Tomosada, L. M., Cox, C. J., Topol, A. B., Vinetz, J. M., & Mayfield, S. (2012). Algae-produced Pfs25 elicits antibodies that inhibit malaria transmission. PloS one, 7(5), e37179.

Figure 1 Recombinant Mouse Anti-Pfs25 Antibody (4B7) in WB.

Western blot analysis of FAMAB-0226-CN was performed by loading recombinant Plasmodium falciparum Pfs25 Antigen (His tag) onto a 15% Tris-HCl polyacrylamide gel. Proteins were transferred to an NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with FAMAB-0226-CN and HRP goat anti-mouse IgG was used as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.5 μg)
Lane 2: Reduced antigen (1.0 μg)
Lane 3: Reduced antigen (1.5 μg)

Figure 2 Recombinant Mouse Anti-Pfs25 Antibody (4B7) in DB.

Dot Blot analysis of FAMAB-0226-CN was performed by coating with Recombinant Plasmodium falciparum Pfs25 Antigen (His tag).
Dot 1: 0.5 μg
Dot 2: 1.0 μg
Dot 3: 1.5 μg

Figure 3 Recombinant Mouse Anti-Pfs25 Antibody (4B7) in ELISA.

ELISA analysis of FAMAB-0226-CN was performed by coating with recombinant Plasmodium falciparum Pfs25 Antigen (His tag). Then blocked with BSA and incubated with FAMAB-0226-CN. The HRP-conjugated goat anti-mouse IgG as a secondary antibody.
Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.