Rabbit Anti-PGM1 Recombinant Antibody (clone R08-9G3)
CAT#: VS3-FY1829
Clone R08-9G3 is a rabbit recombinant monoclonal antibody that specifically directed against human PGM1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. This PGM1 antibody has been rigorously validated and is widely used in western blot analysis of PGM1 in C6, 3T3, Hela lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-PGM1 Antibody (VS3-FY1829) in WB.
The PGM1 expression in C6, 3T3, Hela lysates were detected using the anti-PGM1 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46329/2057_G6_4_selected.jpg
Normal Tissue
Figure 2 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46329/114561_A_9_4.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46329/114561_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46329/114561_A_4_6.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079739-PGM1
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Specifications
- Immunogen
- Synthetic peptide of human PGM1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PGM1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-9G3
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 61 kDa, while the observed molecular weight stands at 61 kDa.
- Related Disease
- Glycogen storage disease type 14
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Phosphoglucomutase 1; Glucose Phosphomutase 1; EC 5.4.2.2; PGM 1; Phosphoglucomutase-1; EC 5.4.2; CDG1T; GSD14
- Gene ID
- 5236
- UniProt ID
- P36871
- Sequence Similarities
- Belongs to the phosphohexose mutase family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylation at Thr-467 by PAK1 significantly enhances enzymatic activity. (P36871-PGM1_HUMAN)
Ubiquitination at Lys146, Lys349, and Lys419 (NX_P36871 [NX_P36871-1])
Modification sites at PhosphoSitePlus (P36871)
Glycosylation from GlyGen (P36871) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001166289.1; NP_001166290.1; NP_002624.2
- Function
- This enzyme participates in both the breakdown and synthesis of glucose.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PGM1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1894MZ | Recombinant Mouse Anti-Human PGM1 Antibody | ICC, IF, IP, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1629 | Anti-PGM1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5350 | Anti-Mouse PGM1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5349 | Anti-Human PGM1 Immunohistochemistry Kit | IHC |
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