Mouse Anti-PHF8 Recombinant Antibody (VS3-CJ15)
CAT#: VS3-CJ15
This product is a mouse antibody that recognizes PHF8.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62015/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38779/81466_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38779/81466_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38779/81466_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38779/81466_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38779/81466_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172943-PHF8
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Specifications
- Immunogen
- Recombinant fragment of human PHF8 (C-terminus)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PHF8
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- KDM7B; JHDM1F; MRXSSD; ZNF422
- Gene ID
- 23133
- UniProt ID
- Q9UPP1
- Sequence Similarities
- Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylation at Ser-69 and Ser-120 are required for dissociation from chromatin and accumulation of H4K20Me1 levels during prophase.
- Protein Refseq
- NP_001171825.1; NP_001171826.1; NP_055922.1
- Function
- Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "PHF8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2452CT | Recombinant Mouse anti-Human PHF8 Monoclonal antibody (nBcdbn) | IHC-P |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2119-CN | Rabbit Anti-PHF8 Polyclonal Antibody (MRO-2119-CN) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-431C | Human Anti-PHF8 Recombinant Antibody (ZG-431C) | ELISA, IP | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-XY3463 | Rabbit Anti-PHF8 Recombinant Antibody (clone R08-1C9) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1641 | Anti-PHF8 Immunohistochemistry Kit | IHC |
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