Rabbit Anti-PHF8 Recombinant Antibody (clone R08-1C9) (CAT#: VS3-XY3463)
It is a recombinant rabbit antibody targeting the PHF8 (PHD finger protein 8), which involves in cell cycle progression by being required to control G1-S transition. The antibody specifically reacts with human, mouse and rat PHF8. It has been determined using Western Blot, Immunoprecipitation, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunoprecipitation and Immunofluorescence is 1/500-1/1000, 1/20 and 1/50-1/200, respectively.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62015/if_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62015/if_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38779/81466_B_9_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38779/81466_B_9_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38779/81466_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38779/81466_A_9_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38779/81466_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38779/81466_A_8_5.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38779/81466_A_4_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38779/81466_A_4_6.jpg
Figure 6 Lymph node
(Germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38779/81466_A_9_8.jpg
(Germinal center cells Staining: Low Intensity: Weak Quantity:>75% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38779/81466_A_9_8.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172943-PHF8
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172943-PHF8
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PHF8
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-1C9
- Applications
- WB, ICC, IF, IP
- Related Disease
- Intellectual Developmental Disorder, X-Linked, Syndromic, Siderius Type; Syndromic X-Linked Intellectual Disability Siderius Type
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- KDM7B; JHDM1F; MRXSSD; ZNF422
- Gene ID
- 23133
- UniProt ID
- Q9UPP1
- Long Name
- PHD finger protein 8
- Cellular Localization
- Nucleus, nucleolus
- Post Translation Modifications
- Phosphorylation at Ser-69 and Ser-120 are required for dissociation from chromatin and accumulation of H4K20Me1 levels during prophase. (Q9UPP1-PHF8_HUMAN)
Ubiquitination at Lys467, Lys486, and Lys732 (NX_Q9UPP1 [NX_Q9UPP1-1])
Modification sites at PhosphoSitePlus (Q9UPP1)
Glycosylation from GlyGen (Q9UPP1) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005262056.1; XP_005262053.1
- Function
- Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development.
Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1).
Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks.
Involved in cell cycle progression by being required to control G1-S transition.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2452CT | Recombinant Mouse anti-Human PHF8 Monoclonal antibody (nBcdbn) | IHC-P | |
| MRO-2119-CN | Rabbit Anti-PHF8 Polyclonal Antibody (MRO-2119-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-431C | Human Anti-PHF8 Recombinant Antibody (ZG-431C) | ELISA, IP | Human IgG1, κ |
| VS3-CJ15 | Mouse Anti-PHF8 Recombinant Antibody (VS3-CJ15) | WB, ELISA | Mouse IgG |
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