Rabbit Anti-Phospho-NFKBIA (S32) Recombinant Antibody (clone 2D6) (CAT#: ZG-0485U)
Rabbit Anti-Phospho-NFKBIA (S32) Antibody (clone 2D6) displayed high specificity against the NFKBIA protein of Human.
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WB
Figure 1 Rabbit Anti-Phospho-NFKBIA (S32) Antibody (ZG-0485U) in WB
Western Blot
Positive WB detected in A549 whole cell lysate(treated with Calyculin A or not)
All lanes Phospho-NFKBIA antibody at 1.07μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 KDa
Observed band size: 39 KDa
IF
Figure 2 Rabbit Anti-Phospho-NFKBIA (S32) Antibody (ZG-0485U) in IF
Immunofluorescence staining of A549 cells with ZG-0485U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-NFKBIA (S32)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-NFKBIA (S32)
- Species Reactivity
- Human
- Clone
- 2D6
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Ectodermal Dysplasia And Immunodeficiency 2 and Hypohidrotic Ectodermal Dysplasia With Immunodeficiency.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; IKBA; MAD3; NFKBI
- Gene ID
- 4792
- UniProt ID
- P25963
Related Resources
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2435 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (DS2435AB) | ICC, IP, WB | IgG |
| MOR-4603 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH116DS) | WB, IHC-P | IgG |
| MOR-4604 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH117DS) | WB | IgG |
| MOR-4686 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH200DS) | WB, FC | IgG |
| MOR-0076-FY | Rabbit Anti-NFKBIA Recombinant Antibody (clone AFY0047) | IF, IHC-P, WB | Rabbit IgG |
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