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    Rabbit Anti-Phospho-NFKBIA (S32) Recombinant Antibody (clone 2D6) (CAT#: ZG-0485U)

    Rabbit Anti-Phospho-NFKBIA (S32) Antibody (clone 2D6) displayed high specificity against the NFKBIA protein of Human.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    We require custom production
    • Tested Data
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    WB

    Figure 1 Rabbit Anti-Phospho-NFKBIA (S32) Antibody (ZG-0485U) in WB

    Figure 1 Rabbit Anti-Phospho-NFKBIA (S32) Antibody (ZG-0485U) in WB

    Western Blot
    Positive WB detected in A549 whole cell lysate(treated with Calyculin A or not)
    All lanes Phospho-NFKBIA antibody at 1.07μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 39 KDa
    Observed band size: 39 KDa

    IF

    Figure 2 Rabbit Anti-Phospho-NFKBIA (S32) Antibody (ZG-0485U) in IF

    Figure 2 Rabbit Anti-Phospho-NFKBIA (S32) Antibody (ZG-0485U) in IF

    Immunofluorescence staining of A549 cells with ZG-0485U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).


    Specifications

    • Immunogen
    • A synthesized peptide derived from human Phospho-NFKBIA (S32)
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human Phospho-NFKBIA (S32)
    • Species Reactivity
    • Human
    • Clone
    • 2D6
    • Applications
    • ELISA, WB, IF
    • Conjugate
    • Unconjugated
    • Related Disease
    • Ectodermal Dysplasia And Immunodeficiency 2 and Hypohidrotic Ectodermal Dysplasia With Immunodeficiency.

    Product Property

    • Purification
    • Affinity chromatography purified
    • Buffer
    • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
    • Preservative
    • 0.02% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence. Specific conditions should be optimized by the end user.
      WB:1:500-1:5000, IF:1:20-1:200

    Target

    • Alternative Names
    • NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; IKBA; MAD3; NFKBI

    Related Resources

    • Related Signaling Pathways

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "NFKBIA"

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    CAT Product Name Application Type
    MOR-2435 Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (DS2435AB) ICC, IP, WB IgG
    MOR-4603 Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH116DS) WB, IHC-P IgG
    MOR-4604 Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH117DS) WB IgG
    MOR-4686 Hi-Affi™ Recombinant Rabbit Anti-NFKBIA Monoclonal Antibody (TH200DS) WB, FC IgG
    MOR-0076-FY Rabbit Anti-NFKBIA Recombinant Antibody (clone AFY0047) IF, IHC-P, WB Rabbit IgG

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