Human Anti-Phosphotyrosine Recombinant Antibody (clone PY20)
CAT#: FAMAB-1527CQ
This product is a recombinant human antibody that recognizes Phosphotyrosine. The antibody was purified by affinity chromatography. It can be used in IHC-P, IP, WB, IF.
Specifications
- Immunogen
- Phosphotyrosine conjugated to Keyhole limpet hemocyanin (KLH)
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Phospho-tyrosine and phosphotyrosine containing proteins
- Clone
- PY20
- Applications
- IHC-P, IP, WB, IF
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Phosphotyrosine; pTyr
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Excellent Reactivity
I am impressed with the anti-phosphotyrosine recombinant antibody clone PY20. It works well in IHC-P, IP, WB and IF applications. The affinity purification process results in a highly specific reactivity to phosphotyrosine residues. The product details and precise instructions ensure consistency in repeated experiments.
Solid Performance
This antibody has been a great addition to our lab for detecting phosphorylation changes. The clone PY20 shows strong performance in multiple assays, including WB and immunofluorescence. The provided documentation is very detailed, assisting with optimal antibody concentration and handling protocols for superior reproducibility.
Q&As
-
How suitable is the PY20 antibody for immunofluorescence applications?
A: We have optimized the anti-phosphotyrosine antibody clone PY20 for multiple applications including immunofluorescence. Its reactivity against phosphotyrosine residues ensures strong signal detection. Detailed purification by affinity chromatography and quality checks guarantee that it performs reliably in immunofluorescence as well as other techniques such as WB, IP and IHC-P.
-
What quality control procedures were followed for PY20?
A: We conduct rigorous quality control on our PY20 antibody using both SDS-PAGE and specific activity assays. This ensures that the antibody displays excellent purity and affinity. The product documentation details the purification process, which further supports its robust performance in WB, IF, IHC-P, and IP experiments.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone PY20"
- CAT
- Product Name
See other products for "phosphotyrosine"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2696 | Hi-Affi™ Recombinant Rabbit Anti-Phosphotyrosine Monoclonal Antibody (DS2696AB) | WB, IP, ICC, IHC, FC, ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-386 | Human Anti-Phosphotyrosine Recombinant Antibody (PABC-386) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-389 | Human Anti-Phosphotyrosine Recombinant Antibody (PABC-389) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-390 | Human Anti-Phosphotyrosine Recombinant Antibody (PABC-390) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-386 | Human Anti-Phosphotyrosine Recombinant Antibody; Fab Fragment (PFBC-386) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-389 | Human Anti-Phosphotyrosine Recombinant Antibody; Fab Fragment (PFBC-389) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-390 | Human Anti-Phosphotyrosine Recombinant Antibody; Fab Fragment (PFBC-390) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-386 | Human Anti-Phosphotyrosine Recombinant Antibody; scFv Fragment (PSBC-386) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-389 | Human Anti-Phosphotyrosine Recombinant Antibody; scFv Fragment (PSBC-389) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-390 | Human Anti-Phosphotyrosine Recombinant Antibody; scFv Fragment (PSBC-390) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1220-CN | Recombinant Rabbit Anti-Phosphotyrosine Monoclonal Antibody (JA10-49) | WB, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1526CQ | Mouse Anti-Phosphotyrosine Recombinant Antibody (clone 4.G10) | IP, WB | Mouse IgG1 |
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