Rabbit Anti-PICK1 Recombinant Antibody (clone R01-4D1)
CAT#: VS3-FY1832
This R01-4D1 recombinant monoclonal antibody exhibits a specific binding affinity towards human PICK1. The antibody is recommended for detection of PICK1 protein by WB, IP. The PICK1 antibody is recommended for detection of target protein by western blot analysis of PICK1 in K562, Hela lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-PICK1 Antibody (VS3-FY1832) in WB.
The PICK1 expression in K562, Hela lysates were detected using the anti-PICK1 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/819/1909_D1_3_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72674/162477_B_9_5.jpg
Normal Tissue
Figure 3 Parathyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72674/162477_A_3_8.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72674/162477_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72674/162477_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100151-PICK1
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Specifications
- Immunogen
- Synthetic peptide of human PICK1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PICK1
- Species Reactivity
- Human
- Clone
- R01-4D1
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 47 kDa, while the observed molecular weight stands at 50 kDa.
- Related Disease
- Spermatogenic Failure 9 and Spermatogenic Failure 5
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Protein Interacting With PRKCA 1; Protein Kinase C-Alpha-Binding Protein; Protein Interacting With C Kinase 1; PRKCABP; Protein Kinase C, Alpha Binding Protein; Protein Interacting With PRKCA; PRKCA-Binding Protein; PICK
- Gene ID
- 9463
- UniProt ID
- Q9NRD5
- Cellular Localization
- Cytoplasm, Cytoskeleton, Membrane, Synapse, Synaptosome
- Post Translation Modifications
- Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors. (Q9NRD5-PICK1_HUMAN)
Palmitoylation on Cys-413 is essential for long-term synaptic depression (LTD). (Q9NRD5-PICK1_HUMAN)
Ubiquitination at Lys209 (NX_Q9NRD5 [NX_Q9NRD5-1])
Modification sites at PhosphoSitePlus (Q9NRD5)
- Protein Refseq
- XP_011528819.1; XP_047297565.1; XP_047297566.1; XP_047297567.1; XP_047297568.1
- Function
- Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competitive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PICK1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1590CT | Recombinant Mouse anti-Human PICK1 Monoclonal antibody (EML993) | WB | |
| VS-0522-LC9 | Mouse Anti-PICK1 Recombinant Antibody (VS-0522-LC9) | IB, IHC, IP | Mouse IgG2a |
| VS-0522-LC10 | Rat Anti-PICK1 Recombinant Antibody (VS-0522-LC10) | IB, IHC, IP | Rat IgG2b |
| VS-0522-LC11 | Human Anti-PICK1 Recombinant Antibody (VS-0522-LC11) | IB, IHC, IP | Human IgG1 |
| VS-0522-LC12 | Rabbit Anti-PICK1 Recombinant Antibody (VS-0522-LC12) | IB, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0795MZ | Chicken Anti-PICK1 Polyclonal IgY | ICC, IF, IHC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2704 | Hi-Affi™ Recombinant Rabbit Anti-PICK1 Monoclonal Antibody (DS2704AB) | WB, IP, FC | IgG |
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