Rabbit Anti-PIK3CD Recombinant Antibody (clone R06-7A9)
CAT#: VS3-XY3465
This is a primary rabbit antibody recognizing with PIK3CD (Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta), which plays a role in B-cell development, proliferation, migration, and function. The antibody specifically reacts with human, mouse and rat PIK3CD. It has been tested using Western Blot, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western blot and Immunofluorescence is 1/500-1/1000 and 1/50-1/200, respectively.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytokinetic bridge & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44953/847_C11_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15420/ihc_selected.jpg
Normal Tissue
Figure 3 Testis
Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44953/139742_A_6_6.jpg
Normal Tissue
Figure 4 Appendix
Lymphoid tissue Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44953/139742_A_3_2.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44953/139742_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, NB-4, REH, THP-1, U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171608-PIK3CD
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PIK3CD
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-7A9
- Applications
- WB, ICC, IF
- Related Disease
- Immunodeficiency 14A With Lymphoproliferation, Autosomal Dominant; Immunodeficiency 14B, Autosomal Recessive
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
Target
- Alternative Names
- APDS; PI3K; IMD14; p110D; IMD14A; IMD14B; ROCHIS; P110DELTA
- Gene ID
- 5293
- UniProt ID
- O00329
- Long Name
- Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity. (O00329-PK3CD_HUMAN)
Ubiquitination at Lys46 and Lys841 (NX_O00329 [NX_O00329-1])
Modification sites at PhosphoSitePlus (O00329)
Glycosylation from GlyGen (O00329) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_006710750.1
- Function
- Mediates immune responses.
Plays a role in B-cell development, proliferation, migration, and function.
Required for B-cell receptor (BCR) signaling.
Mediates B-cell proliferation response to anti-IgM, anti-CD40 and IL4 stimulation.
Promotes cytokine production in response to TLR4 and TLR9.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PIK3CD"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1368z | Mouse Anti-PIK3CD Recombinant Antibody (clone 24D11) | WB, IP, IF, IHC, ELISA | Mouse IgG1, κ |
| MOB-2345CT | Recombinant Mouse anti-Human PIK3CD Monoclonal antibody (2H3) | WB, ICC | |
| MRO-1223-CN | Recombinant Rabbit Anti-PIK3CD Monoclonal Antibody (JM83-24) | WB, IHC | Rabbit IgG |
| VS3-WK615 | Rabbit Anti-PIK3CD Recombinant Antibody (clone JM83-24) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2709 | Hi-Affi™ Recombinant Rabbit Anti-PIK3CD Monoclonal Antibody (DS2709AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5407 | Anti-PIK3CD Immunohistochemistry Kit | IHC | |
| VS-0525-XY5408 | Anti-Mouse PIK3CD Immunohistochemistry Kit | IHC |
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