Rabbit Anti-PIN4 Recombinant Antibody (clone R06-8I5)
CAT#: VS3-FY1833
Clone R06-8I5 is a rabbit recombinant monoclonal antibody that specifically directed against human PIN4. It cross-reacts with rat, hamster. It is suitable for use in a variety of assays, including WB, IP. The PIN4 antibody is recommended for detection of target protein by western blot analysis of PIN4 in CHO-K1, C6, Jurkat lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54483/870_C9_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64504/1193_G11_1_selected.jpg
Normal Tissue
Figure 3 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows moderate cytoplasmic and nuclear positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54483/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows nuclear and cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64504/169178_B_2_6_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64504/169178_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64504/169178_A_7_3.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64504/169178_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000102309-PIN4
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WB
Figure 1 Anti-PIN4 Antibody (VS3-FY1833) in WB.
The PIN4 expression in CHO-K1, C6, Jurkat lysates were detected using the anti-PIN4 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human PIN4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat, Hamster PIN4
- Species Reactivity
- Human, Rat, Hamster
- Clone
- R06-8I5
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 14 kDa, while the observed molecular weight stands at 14 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Peptidylprolyl Cis/Trans Isomerase, NIMA-Interacting 4; Peptidyl-Prolyl Cis-Trans Isomerase Pin4; Peptidyl-Prolyl Cis/Trans Isomerase EPVH; Parvulin-14; Parvulin-17; Parvulin; HPar14; HPar17; PAR14; PAR17; HEPVH; Protein (Peptidyl-Prolyl Cis/Trans Isomerase) NIMA-Interacting, 4 (Parvulin)
- Gene ID
- 5303
- UniProt ID
- Q9Y237
- Sequence Similarities
- Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus
- Post Translation Modifications
- Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus., Isoform 2 is sumoylated with SUMO2 and SUMO3.
- Protein Refseq
- NP_001164218.1; NP_006214.2
- Function
- Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.
Isoform 2 binds to double-stranded DNA.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PIN4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2716 | Hi-Affi™ Recombinant Rabbit Anti-PIN4 Monoclonal Antibody (DS2716AB) | WB, IHC-P, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS4-CJ76 | Recombinant Rabbit Anti-PIN4 Antibody (clone 14A7) | ELISA, WB, IHC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1654 | Anti-PIN4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5428 | Anti-Mouse PIN4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5427 | Anti-Human PIN4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5429 | Anti-Rat PIN4 Immunohistochemistry Kit | IHC |
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