Rabbit Anti-PIN4 Recombinant Antibody (clone R06-8I5) (CAT#: VS3-FY1833)
Clone R06-8I5 is a rabbit recombinant monoclonal antibody that specifically directed against human PIN4. It cross-reacts with rat, hamster. It is suitable for use in a variety of assays, including WB, IP. The PIN4 antibody is recommended for detection of target protein by western blot analysis of PIN4 in CHO-K1, C6, Jurkat lysates.
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WB
Figure 1 Anti-PIN4 Antibody (VS3-FY1833) in WB.
The PIN4 expression in CHO-K1, C6, Jurkat lysates were detected using the anti-PIN4 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human PIN4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat, Hamster PIN4
- Species Reactivity
- Human, Rat, Hamster
- Clone
- R06-8I5
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 14 kDa, while the observed molecular weight stands at 14 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Peptidylprolyl Cis/Trans Isomerase, NIMA-Interacting 4; Peptidyl-Prolyl Cis-Trans Isomerase Pin4; Peptidyl-Prolyl Cis/Trans Isomerase EPVH; Parvulin-14; Parvulin-17; Parvulin; HPar14; HPar17; PAR14; PAR17; HEPVH; Protein (Peptidyl-Prolyl Cis/Trans Isomerase) NIMA-Interacting, 4 (Parvulin)
- Gene ID
- 5303
- UniProt ID
- Q9Y237
- Sequence Similarities
- Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus
- Post Translation Modifications
- Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus., Isoform 2 is sumoylated with SUMO2 and SUMO3.
- Protein Refseq
- NP_001164218.1; NP_006214.2
- Function
- Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.
Isoform 2 binds to double-stranded DNA.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2716 | Hi-Affi™ Recombinant Rabbit Anti-PIN4 Monoclonal Antibody (DS2716AB) | WB, IHC-P, ICC, IP | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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