Recombinant Rabbit Anti-PIN4 Antibody (clone 14A7) (CAT#: VS4-CJ76)
This rabbit anti-PIN4 antibody (clone 14A7) is a product that recognizes human PIN4. The immunogen of this antibody is a synthesized peptide derived from human PIN4. The mAb has been tested for use in ELISA, Western Blot (1:500-1:2000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54483/870_C9_2_selected.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54483/870_C9_2_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64504/1193_G11_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64504/1193_G11_1_selected.jpg
Figure 3 IHC staining of human esophagus
(Immunohistochemical staining of human esophagus shows moderate cytoplasmic and nuclear positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54483/ihc_selected.jpg
(Immunohistochemical staining of human esophagus shows moderate cytoplasmic and nuclear positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54483/ihc_selected.jpg
Figure 4 IHC staining of human skeletal muscle
(Immunohistochemical staining of human skeletal muscle shows nuclear and cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64504/169178_B_2_6_selected.jpg
(Immunohistochemical staining of human skeletal muscle shows nuclear and cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64504/169178_B_2_6_selected.jpg
Figure 5 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64504/169178_B_9_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64504/169178_B_9_5.jpg
Figure 6 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64504/169178_A_7_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64504/169178_A_7_3.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64504/169178_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64504/169178_A_6_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000102309-PIN4
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000102309-PIN4
Specifications
- Immunogen
- A synthesized peptide derived from human PIN4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PIN4
- Species Reactivity
- Human
- Clone
- 14A7
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:2000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200).
Target
- Alternative Names
- EPVH; PAR14; PAR17; hEPVH; hPar14; hPar17
- Gene ID
- 5303
- UniProt ID
- Q9Y237
- Long Name
- Peptidylprolyl cis/trans isomerase, NIMA-interacting 4
- Sequence Similarities
- Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus
- Post Translation Modifications
- Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus.
Isoform 2 is sumoylated with SUMO2 and SUMO3.
- Protein Refseq
- NP_001164218.1; NP_006214.2
- Function
- Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.
Isoform 2 binds to double-stranded DNA.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2716 | Hi-Affi™ Recombinant Rabbit Anti-PIN4 Monoclonal Antibody (DS2716AB) | WB, IHC-P, ICC, IP | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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