Rabbit Anti-PKN1 Recombinant Antibody (clone R09-2I4) (CAT#: VS3-FY1836)
This anti-PKN1 antibody detected positive in western blot analysis of PKN in C6 lysates. The recombinant monoclonal antibody exhibits a specific binding affinity towards human and rat PKN1. The R09-2I4 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000).
Specific Inquiry
WB
Figure 1 Anti-PKN1 Antibody (VS3-FY1836) in WB.
The PKN expression in C6 lysates were detected using the anti-PKN1 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human PKN
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat PKN1
- Species Reactivity
- Human, Rat
- Clone
- R09-2I4
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 104 kDa, while the observed molecular weight stands at 120 kDa.
- Related Disease
- Mevalonic Aciduria and Malignant Acrospiroma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
- Gene ID
- 5585
- UniProt ID
- Q16512
- Sequence Similarities
- Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Endosome, Membrane, Nucleus
- Post Translation Modifications
- Autophosphorylated; preferably on serine.
Phosphorylated during mitosis.
(Q16512-PKN1_HUMAN)
Activated by limited proteolysis with trypsin. (Q16512-PKN1_HUMAN)
(Microbial infection) In case of infection, polyubiquitinated by the bacterial E3 ubiquitin-protein ligase SspH1, leading to its proteasomal degradation. (Q16512-PKN1_HUMAN)
Glycosylation at Asn154 (NX_Q16512 [NX_Q16512-1])
Ubiquitination at Lys263 (NX_Q16512 [NX_Q16512-1])
Modification sites at PhosphoSitePlus (Q16512)
Glycosylation from GlyConnect PKN1_HUMAN (1737)
Glycosylation from GlyGen (Q16512) 3 sites, 1 N-linked glycan (1 site), 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_002732.3; NP_998725.1
- Function
- PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PKN1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2537z | Mouse Anti-PKN1 Recombinant Antibody (clone 38A5) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG2a |
| MOB-0781MZ | Recombinant Mouse Anti-Human PKN1 Antibody (clone 1B10) | WB | Mouse antibody |
| VS3-QX881 | Mouse Anti-PKN1 Recombinant Antibody (clone 1C12B8) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
| VS3-QX882 | Mouse Anti-PKN1 Recombinant Antibody (clone B1-10H) | WB, IHC, ICC, FC | Mouse IgG2b |
| VS3-XY1263 | Mouse Anti-PKN1 Recombinant Antibody (clone 2D7) | ELISA, WB, FC, ICC, IHC | Mouse IgG2b |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2726 | Hi-Affi™ Recombinant Rabbit Anti-PKN1 Monoclonal Antibody (DS2726AB) | WB, FC | IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-FY1836. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.