Rabbit Anti-PKN1 Recombinant Antibody (clone R09-2I4)
CAT#: VS3-FY1836
This anti-PKN1 antibody detected positive in western blot analysis of PKN in C6 lysates. The recombinant monoclonal antibody exhibits a specific binding affinity towards human and rat PKN1. The R09-2I4 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000).
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytokinetic bridge.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3982/1772_A10_12_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3982/12798_A_9_5_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10278/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3982/12798_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3982/12798_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3982/12798_A_9_4.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3982/12798_A_6_6.jpg
Normal Tissue
Figure 8 Fallopian tube
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3982/12798_A_9_7.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3982/12798_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123143-PKN1
❮
❯
WB
Figure 1 Anti-PKN1 Antibody (VS3-FY1836) in WB.
The PKN expression in C6 lysates were detected using the anti-PKN1 by western blot assay.
❮
❯
Specifications
- Immunogen
- Synthetic peptide of human PKN
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat PKN1
- Species Reactivity
- Human, Rat
- Clone
- R09-2I4
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 104 kDa, while the observed molecular weight stands at 120 kDa.
- Related Disease
- Mevalonic Aciduria and Malignant Acrospiroma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
- Gene ID
- 5585
- UniProt ID
- Q16512
- Sequence Similarities
- Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Endosome, Membrane, Nucleus
- Post Translation Modifications
- Autophosphorylated; preferably on serine.
Phosphorylated during mitosis.
(Q16512-PKN1_HUMAN)
Activated by limited proteolysis with trypsin. (Q16512-PKN1_HUMAN)
(Microbial infection) In case of infection, polyubiquitinated by the bacterial E3 ubiquitin-protein ligase SspH1, leading to its proteasomal degradation. (Q16512-PKN1_HUMAN)
Glycosylation at Asn154 (NX_Q16512 [NX_Q16512-1])
Ubiquitination at Lys263 (NX_Q16512 [NX_Q16512-1])
Modification sites at PhosphoSitePlus (Q16512)
Glycosylation from GlyConnect PKN1_HUMAN (1737)
Glycosylation from GlyGen (Q16512) 3 sites, 1 N-linked glycan (1 site), 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_002732.3; NP_998725.1
- Function
- PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY1836. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PKN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2537z | Mouse Anti-PKN1 Recombinant Antibody (clone 38A5) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG2a |
| VS3-QX881 | Mouse Anti-PKN1 Recombinant Antibody (clone 1C12B8) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
| VS3-QX882 | Mouse Anti-PKN1 Recombinant Antibody (clone B1-10H) | WB, IHC, ICC, FC | Mouse IgG2b |
| VS13-YC905 | Rabbit Anti-PKN1 Recombinant Antibody (VS13-YC905) | WB | Rabbit IgG |
| VS7-0425-WR621 | Mouse Anti-PKN1 Recombinant Antibody (clone 4H10B1) | IHC, ICC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2726 | Hi-Affi™ Recombinant Rabbit Anti-PKN1 Monoclonal Antibody (DS2726AB) | WB, FC | IgG |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, ELISA, FuncS, IB, FC, SPR, Apop
Application: ELISA, WB, FC
Application: ELISA, Neut
Application: ELISA, Inhib, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
