Rabbit Anti-PON1 Recombinant Antibody (clone R07-4I1)
CAT#: VS3-FY1840
The R07-4I1 recombinant monoclonal antibody is specific for human PON1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IP. The specificity of the PON1 antibody was confirmed by western blot analysis of PON1 in mouse liver lysates.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IHC staining of human liver
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1610/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1610/6964_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1610/6964_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1610/6964_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1610/6964_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1610/6964_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1610/6964_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Not detected
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000005421-PON1/subcellular
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WB
Figure 1 Anti-PON1 Antibody (VS3-FY1840) in WB.
The PON1 expression in mouse liver lysates were detected using the anti-PON1 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human PON1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PON1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-4I1
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 40 kDa, while the observed molecular weight stands at 40 kDa.
- Related Disease
- Microvascular Complications Of Diabetes 5 and Amyotrophic Lateral Sclerosis 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- PON1; ESA; MVCD5; PON; paraoxonase 1
- Gene ID
- 5444
- UniProt ID
- P27169
- Sequence Similarities
- Belongs to the paraoxonase family.
- Cellular Localization
- HDL, Secreted
- Post Translation Modifications
- Glycosylated. (P27169-PON1_HUMAN)
The signal sequence is not cleaved. (P27169-PON1_HUMAN)
Present in two forms, form B contains a disulfide bond, form A does not. (P27169-PON1_HUMAN)
Glycosylation at Asn227, Asn253, Asn270, and Asn324 (NX_P27169 [NX_P27169-1])
Modification sites at PhosphoSitePlus (P27169)
Glycosylation from GlyConnect PON1_HUMAN (726)
Glycosylation from GlyGen (P27169) 6 sites, 30 N-linked glycans (3 sites), 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_000437.3
- Function
- Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PON1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1710z | Mouse Anti-PON1 Recombinant Antibody (clone 22G10) | WB, ELISA, FC, IHC | Mouse IgG1 |
| MOB-2409CT | Recombinant Mouse anti-Human PON1 Monoclonal antibody (FQ3048BZ) | ICC, IF, IHC-FoFr, ELISA, WB, FC | |
| VS3-XY1276 | Mouse Anti-PON1 Recombinant Antibody (clone 4G8D3) | IHC, ICC | Mouse IgG1 |
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| VS-0923-FY136 | Recombinant Mouse Anti-PON1 Antibody (VS-0923-FY136) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2788 | Hi-Affi™ Recombinant Rabbit Anti-PON1 Monoclonal Antibody (DS2788AB) | IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
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| VS-0724-YC327 | AbPlus™ Anti-PON1 Magnetic Beads (VS-0724-YC327) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1700 | Anti-PON1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5569 | Anti-Mouse PON1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5568 | Anti-Human PON1 Immunohistochemistry Kit | IHC |
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