Rabbit Anti-POR Recombinant Antibody (clone R05-9D4)
CAT#: VS3-FY1692
The R05-9D4 recombinant monoclonal antibody binds to human POR. It cross-reacts with mouse, rat. The antibody is recommended for detection of POR protein by WB, IHC-F, IHC-P, ICC/IF. The binding specificity of the cytochrome P450 reductase antibody was determined using western blot analysis of cytochrome P450 reductase in K562, rat brain, C6, 3T3 lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-POR Antibody (VS3-FY1692) in WB.
The Cytochrome P450 Reductase expression in K562, rat Brain, C6, 3T3 lysates were detected using the anti-Cytochrome P450 Reductase (POR) by western blot assay.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10136/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human liver
Immunohistochemical staining shows high expression in liver
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10136/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4372/12665_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4372/12665_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4372/12665_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4372/12665_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4372/12665_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4372/12665_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000127948-POR/subcellular
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Specifications
- Immunogen
- Synthetic peptide of human cytochrome P450 reductase
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat POR
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-9D4
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 77 kDa, while the observed molecular weight stands at 77 kDa.
- Related Disease
- Disordered Steroidogenesis Due To Cytochrome P450 Oxidoreductase Deficiency and Antley-Bixler Syndrome With Genital Anomalies And Disordered Steroidogenesis
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Cytochrome P450 Reductase; POR; cytochrome p450 oxidoreductase; Cytochrome P450 Reductase
- Gene ID
- 5447
- UniProt ID
- P16435
- Sequence Similarities
- Belongs to the NADPH--cytochrome P450 reductase family.
- Cellular Localization
- Endoplasmic reticulum, Membrane
- Post Translation Modifications
- Acetylation
Phosphoprotein
- Protein Refseq
- NP_000932.3; NP_001354491.2; NP_001369584.2; NP_001369586.2; NP_001369587.2
- Function
- This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "POR"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2411z | Mouse Anti-POR Recombinant Antibody (clone 15C11) | WB, FC, ICC, IF, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0530CT | Recombinant Mouse anti-Human POR Monoclonal antibody (6I6) | IHC-P, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2791 | Hi-Affi™ Recombinant Rabbit Anti-POR Monoclonal Antibody (DS2791AB) | WB, IHC-P, ICC, IF, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0432-CN | Recombinant Rabbit Anti-POR Monoclonal Antibody (JB54-31) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5572 | Anti-POR Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5573 | Anti-Mouse POR Immunohistochemistry Kit | IHC |
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