Rabbit Anti-POR Recombinant Antibody (clone R05-9D4) (CAT#: VS3-FY1692)

The R05-9D4 recombinant monoclonal antibody binds to human POR. It cross-reacts with mouse, rat. The antibody is recommended for detection of POR protein by WB, IHC-F, IHC-P, ICC/IF. The binding specificity of the cytochrome P450 reductase antibody was determined using western blot analysis of cytochrome P450 reductase in K562, rat brain, C6, 3T3 lysates.

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  • Tested Data
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WB

Figure 1 Anti-POR Antibody (VS3-FY1692) in WB.

Figure 1 Anti-POR Antibody (VS3-FY1692) in WB.

The Cytochrome P450 Reductase expression in K562, rat Brain, C6, 3T3 lysates were detected using the anti-Cytochrome P450 Reductase (POR) by western blot assay.


Specifications

  • Immunogen
  • Synthetic peptide of human cytochrome P450 reductase
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat POR
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R05-9D4
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
  • MW
  • The calculated weight of the target protein is approximately 77 kDa, while the observed molecular weight stands at 77 kDa.
  • Related Disease
  • Disordered Steroidogenesis Due To Cytochrome P450 Oxidoreductase Deficiency and Antley-Bixler Syndrome With Genital Anomalies And Disordered Steroidogenesis

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • Cytochrome P450 Reductase; POR; cytochrome p450 oxidoreductase; Cytochrome P450 Reductase
  • Sequence Similarities
  • Belongs to the NADPH--cytochrome P450 reductase family.
  • Cellular Localization
  • Endoplasmic reticulum, Membrane
  • Post Translation Modifications
  • Acetylation
    Phosphoprotein
  • Function
  • This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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