Mouse Anti-PPP1CA Monoclonal Antibody (VS7-0425-WR662)
CAT#: VS7-0425-WR662
This product is a mouse IgG antibody that binds to human PPP1CA. The antibody can be used for WB and ELISA assays. It cross reacts with mouse.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46833/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46833/133850_A_7_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46833/133850_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46833/133850_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46833/133850_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46833/133850_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46833/133850_A_5_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4545/20830_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172531-PPP1CA/subcellular
❮
❯
Specifications
- Immunogen
- Recombinant human PPP1CA fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PPP1CA
- Species Reactivity
- Human, Mouse
- Molecule Class
- Serine/Threonine Protein Phosphatase
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PP1A; PP-1A; PPP1A; PP1alpha
- Gene ID
- 5499
- UniProt ID
- P62136
- Long Name
- Protein Phosphatase 1 Catalytic Subunit Alpha
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_001008709.1
- Function
- Hydrolase; Protein phosphatase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PPP1CA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1189CT | Recombinant Mouse anti-Human PPP1CA Monoclonal antibody (5H4) | ELISA, WB | |
| VS3-CJ18 | Mouse Anti-PPP1CA Recombinant Antibody (VS3-CJ18) | WB, ELISA | Mouse IgG |
| VS3-XY1288 | Mouse Anti-PPP1CA Recombinant Antibody (clone 1C11-C10-H5-E9) | WB, ICC | Mouse IgG1 |
| VS3-XY1289 | Mouse Anti-PPP1CA Recombinant Antibody (clone 7C7C9) | ELISA, WB | Mouse IgG1 |
| VS3-FY1843 | Rabbit Anti-PPP1CA Recombinant Antibody (clone R04-8E4) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2814 | Hi-Affi™ Recombinant Rabbit Anti-PPP1CA Monoclonal Antibody (DS2814AB) | WB, IP, ICC, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1920 | PE-A*02:01/Human PPP1CA (SIIGRLLEV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1717 | Anti-PPP1CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5616 | Anti-Mouse PPP1CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5615 | Anti-Human PPP1CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5617 | Anti-Rat PPP1CA Immunohistochemistry Kit | IHC |
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