Mouse Anti-PPP2CB Recombinant Antibody (VS3-FY2840)
CAT#: VS3-FY2840
The anti-PPP2CB product is a mouse monoclonal antibody that shows high affinity for human PPP2CB protein. The calculated molecular weight of the protein is about 35.5 kDa. It is recommended for detection of PPP1CC protein by Western Blot (1/500-1/2000), Immunohistochemistry (1/50-1/200), Flow cytometry (1/50-1/200) and ELISA (1/1000-1/10000).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18600/947_A1_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_5_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104695-PPP2CB/subcellular
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PPP2CB
- Species Reactivity
- Human
- Applications
- Western Blot, Immunohistochemistry, Flow cytometry, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 35.5 kDa.
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- PP2CB; PP2Abeta
- Gene ID
- 5516
- Sequence Similarities
- Belongs to the PPP phosphatase family. PP-1 subfamily.
- Cellular Localization
- Cytoplasm
Nucleus
Chromosome, centromere
Cytoplasm, cytoskeleton, spindle pole
- Post Translation Modifications
- May be monoubiquitinated by NOSIP. (P62714-PP2AB_HUMAN)
Ubiquitination at Lys21 (NX_P62714 [NX_P62714-1])
Modification sites at PhosphoSitePlus (P62714)
Glycosylation from GlyGen (P62714) 1 site, 1 O-linked glycan (1 site)
- Function
- Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events (Probable).
PP2A can modulate the activity of phosphorylase B kinase, casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PPP2CB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2826 | Hi-Affi™ Recombinant Rabbit Anti-PPP2CB Monoclonal Antibody (DS2826AB) | WB, ICC, IF, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS1-YJ185 | Mouse Anti-PPP2CB Recombinant Antibody (clone H5D12) | IF, WB, IP | Mouse IgG1 |
| VS1-YJ186 | Mouse Anti-PPP2CB Recombinant Antibody (clone H5D12), HRP | IF, WB, IP | Mouse IgG1 |
| VS3-QX907 | Mouse Anti-PPP2CB Recombinant Antibody (clone A0-G7) | WB, ICC | Mouse IgG1 |
| VS13-YC924 | Rabbit Anti-PPP2CB Recombinant Antibody (VS13-YC924) | WB, ICC, IF, IHC-P, IP | Rabbit IgG |
| VS7-0425-WR667 | Mouse Anti-PPP2CB Monoclonal Antibody (clone 4D2C7) | WB, IHC, FC | Mouse IgG1 |
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