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    Mouse Anti-PPP2CB Recombinant Antibody (VS3-FY2840) (CAT#: VS3-FY2840)

    The anti-PPP2CB product is a mouse monoclonal antibody that shows high affinity for human PPP2CB protein. The calculated molecular weight of the protein is about 35.5 kDa. It is recommended for detection of PPP1CC protein by Western Blot (1/500-1/2000), Immunohistochemistry (1/50-1/200), Flow cytometry (1/50-1/200) and ELISA (1/1000-1/10000).

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/18600/947_A1_1_selected.jpg
    Figure 2 IHC staining of human kidney
    Figure 2 IHC staining of human kidney
    (Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/ihc_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glial cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Neuronal cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Neuropil
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glandular cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_7_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Hepatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_7_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_8_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Cells in seminiferous ducts
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_5_6.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43236/103641_A_8_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104695-PPP2CB/subcellular

    Specifications

    • Host Species
    • Mouse
    • Type
    • Mouse IgG
    • Specificity
    • Human PPP2CB
    • Species Reactivity
    • Human
    • Applications
    • Western Blot, Immunohistochemistry, Flow cytometry, ELISA
    • Conjugate
    • Unconjugated, FITC
    • MW
    • The molecular weight of the target protein is approximately 35.5 kDa.

    Product Property

    • Clonality
    • Monoclonal Antibody
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Western Blot: 1/500-1/2000
      Immunohistochemistry: 1/50-1/200
      Flow cytometry: 1/50-1/200
      ELISA: 1/1000-1/10000

    Target

    • Alternative Names
    • PP2CB; PP2Abeta
    • Sequence Similarities
    • Belongs to the PPP phosphatase family. PP-1 subfamily.
    • Cellular Localization
    • Cytoplasm
      Nucleus
      Chromosome, centromere
      Cytoplasm, cytoskeleton, spindle pole
    • Post Translation Modifications
    • May be monoubiquitinated by NOSIP. (P62714-PP2AB_HUMAN)
      Ubiquitination at Lys21 (NX_P62714 [NX_P62714-1])
      Modification sites at PhosphoSitePlus (P62714)
      Glycosylation from GlyGen (P62714) 1 site, 1 O-linked glycan (1 site)
    • Function
    • Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events (Probable).
      PP2A can modulate the activity of phosphorylase B kinase, casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.

    Recommended Products

    Secondary Antibody

    Isotype Control

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "PPP2CB"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    Rabbit Monoclonal Antibody Products IgG Antibody Products
    CAT Product Name Application Type
    MOR-2826 Hi-Affi™ Recombinant Rabbit Anti-PPP2CB Monoclonal Antibody (DS2826AB) WB, ICC/IF, IP IgG

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