Rabbit Anti-PPP2R5E Recombinant Antibody (clone R06-6E2)
CAT#: VS3-FY1846
The R06-6E2 recombinant monoclonal antibody is specific for human PPP2R5E. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We verify the QC result of the antibody through western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). This PPP2R5E antibody is guaranteed for western blot analysis of PPP2R5E in C6, 3T3, mouse brain, rat brain lysates.
Tested Data
WB
Figure 1 Anti-PPP2R5E Antibody (VS3-FY1846) in WB.
The PPP2R5E expression in C6, 3T3, mouse brain, rat brain lysates were detected using the anti-PPP2R5E by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human PPP2R5E
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PPP2R5E
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-6E2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 55 kDa, while the observed molecular weight stands at 55 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- PPP2R5E; protein phosphatase 2 regulatory subunit B'epsilon; B56epsilon; B56E
- Gene ID
- 5529
- UniProt ID
- Q16537
- Sequence Similarities
- Belongs to the phosphatase 2A regulatory subunit B56 family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylated on serine residues. (Q16537-2A5E_HUMAN)
Modification sites at PhosphoSitePlus (Q16537)
Modification sites at neXtProt (NX_Q16537)
- Protein Refseq
- XP_024305415.1; XP_047287499.1; XP_047287500.1; XP_054232293.1; XP_054232294.1
- Function
- The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PPP2R5E"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC390 | AbPlus™ Anti-PPP2R5E Magnetic Beads (VS-0724-YC390) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5635 | Anti-PPP2R5E Immunohistochemistry Kit | IHC |
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