Rabbit Anti-PPP3CA Recombinant Antibody (clone R09-6K9)
CAT#: VS3-XY3475
This product is a recombinant rabbit antibody binding to PPP3CA (Protein phosphatase 3 catalytic subunit alpha), which positively regulates osteoclast differentiation, potentially via NFATC1 signaling. PPP3CA may play a role in skeletal muscle fiber type specification, potentially via NFATC1 signaling. This antibody specifically reacts with human, mouse and rat PPP3CA. It has been detected by Western Blot, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18581/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12778/42715_B_7_5.jpg
Normal Tissue
Figure 3 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12778/42715_A_6_6.jpg
Normal Tissue
Figure 4 Prostate
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12778/42715_A_3_5.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12778/42715_A_7_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138814-PPP3CA/subcellular
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PPP3CA
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-6K9
- Applications
- WB, ICC, IF, FC
- Related Disease
- Arthrogryposis, Cleft Palate, Craniosynostosis, And Impaired Intellectual Development
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- CALN; CCN1; CNA1; CALNA; DEE91; IECEE; PPP2B; ACCIID; CALNA1; IECEE1
- Gene ID
- 5530
- UniProt ID
- Q08209
- Long Name
- Protein phosphatase 3 catalytic subunit alpha
- Cellular Localization
- Cytoplasm, Cell membrane
- Post Translation Modifications
- Ubiquitination at Lys32 and Lys142 (NX_Q08209 [NX_Q08209-1])
Modification sites at PhosphoSitePlus (Q08209)
Glycosylation from GlyGen (Q08209) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001124163.1; NP_000935.1
- Function
- In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation (PubMed:15671020).
In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion (PubMed:18838687).
Positively regulates the CACNA1B/CAV2.2-mediated Ca(2+) release probability at hippocampal neuronal soma and synaptic terminals (By similarity).
Dephosphorylates heat shock protein HSPB1 (By similarity).
Dephosphorylates and activates transcription factor NFATC1.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PPP3CA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2830 | Hi-Affi™ Recombinant Rabbit Anti-PPP3CA Monoclonal Antibody (DS2830AB) | WB, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0211-CN | Recombinant Rabbit Anti-PPP3CA Monoclonal Antibody (JA64-11) | WB, FC | Rabbit IgG |
| VS3-WK263 | Rabbit Anti-PPP3CA Recombinant Antibody (clone JA64-11) | WB, FC | Rabbit IgG |
| VS3-FY1178 | Recombinant Rabbit Anti-PPP3CA Antibody (clone R01-6F5) | WB, IP | Rabbit IgG |
| VS7-0425-WR669 | Mouse Anti-PPP3CA Recombinant Antibody (VS7-0425-WR669) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1726 | Anti-PPP3CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5636 | Anti-Human PPP3CA Immunohistochemistry Kit | IHC |
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