Rabbit Anti-PRC1 Recombinant Antibody (clone R04-3G7)
CAT#: VS3-FY1848
The R04-3G7 recombinant monoclonal antibody is specific for human PRC1. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-P, IP. This PRC1 antibody has been rigorously validated and is widely used in western blot analysis of PRC1 in C6, Jurkat lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-PRC1 Antibody (VS3-FY1848) in WB.
The PRC1 expression in C6, Jurkat lysates were detected using the anti-PRC1 by western blot assay.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, microtubules & cytokinetic bridge.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34521/1859_G1_34_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:
Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34521/80174_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34521/80174_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34521/80174_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34521/80174_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34521/80174_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34521/80174_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198901-PRC1/subcellular
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Specifications
- Immunogen
- Synthetic peptide of human PRC1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat PRC1
- Species Reactivity
- Human, Rat
- Clone
- R04-3G7
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 72 kDa, while the observed molecular weight stands at 72 kDa.
- Related Disease
- Bladder Cancer and Noonan Syndrome 3
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- ASE1; MAP65
- Gene ID
- 9055
- UniProt ID
- O43663
- Sequence Similarities
- Belongs to the MAP65/ASE1 family.
- Cellular Localization
- Chromosome, Cytoplasm, Cytoskeleton, Microtubule, Nucleus
- Post Translation Modifications
- Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules.
- Protein Refseq
- XP_005255044.1; XP_006720822.1; XP_006720823.1; XP_011520489.1; XP_011520490.1
- Function
- Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PRC1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0315CT | Recombinant Mouse anti-Saccharomyces cerevisiae PRC1 Monoclonal antibody (20B6C6) | ICC, IF, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2834 | Hi-Affi™ Recombinant Rabbit Anti-PRC1 Monoclonal Antibody (DS2834AB) | IHC-P, WB, IHC-P, ICC, IF, FC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK2011 | Rabbit Anti-PRC1 (pT481) Recombinant Antibody (clone R06-2A3) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5649 | Anti-PRC1 Immunohistochemistry Kit | IHC |
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