Anti-PRDM1 Immunohistochemistry Kit

Name: Anti-PRDM1 Immunohistochemistry Kit
SKU: VS-0525-XY5650
Rating: 5 (1 reviews)

CAT#: VS-0525-XY5650

The PRDM1 IHC kit offers complete reagents for detecting PRDM1 in tissue samples. It is optimized for use on both paraffin and frozen tissue sections, ensuring reliable, high-quality results for your research.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30033/if_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human tonsil

Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center and non-germinal center cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/75427/168778_A_5_8_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30033/323_D2_3_blue_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Glial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/75427/168778_B_9_5.jpg

Normal Tissue Figure 5 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/75427/168778_A_7_3.jpg

Normal Tissue Figure 6 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/75427/168778_A_7_4.jpg

Normal Tissue Figure 7 Kidney

Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/75427/168778_A_8_5.jpg

Normal Tissue Figure 8 Testis

Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/75427/168778_A_6_6.jpg

Normal Tissue Figure 9 Lymph node

Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/75427/168778_A_8_8.jpg

Normal Tissue Figure 10 Tonsil

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/75427/168778_A_4_8.jpg

RNA Expression Figure 11 RNA cell line category: Cell line enhanced (Karpas-707, RPMI-8226, U-266/70, U-266/84)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000057657-PRDM1

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Specifications

Application
IHC

Size
50 Tests

Species Reactivity
Human

Target
PRDM1

Primary Antibody
Mouse Anti-PRDM1 Antibody

Secondary Antibody
Goat anti-Mouse Antibody, HRP

Sample Type
FFPE tissue; Frozen section tissue

Kit Storage
All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Datasheet

Datasheet - VS-0525-XY5650.pdf
IHC Kits-FFPE Protocol.pdf
IHC Kits-FS Protocol.pdf

MSDS

MSDS - VS-0525-XY5650.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "PRDM1"

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DrMAB-470	Recombinant Human Anti-Human PRDM1 Antibody, clone D602	ELISA, IP-MS	IgG

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MOB-1681CT	Recombinant Mouse anti-Mouse PRDM1 Monoclonal antibody (4I3-F9)	ICC, IF, IHC-P, WB

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