Mouse Anti-PRF1 Recombinant Antibody (clone 6F8C2)
CAT#: VS7-0425-WR680
This product is a mouse IgG1 antibody that binds to human PRF1. The antibody 6F8C2 recognizes specific amino acid domain of human PRF1 (AA: extra 148-372). It can be used for FC assay.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37940/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2436/ihc_selected.jpg
Normal Tissue
Figure 3 Spleen
Cells in red pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2436/6381_B_8_4.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enriched (HDLM-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180644-PRF1/subcellular
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Specifications
- Immunogen
- Recombinant human PRF1 (AA: extra 148-372) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PRF1
- Species Reactivity
- Human
- Clone
- 6F8C2
- Molecule Class
- Pore-Forming Protein
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Hemophagocytic lymphohistiocytosis, familial, 2 (FHL2)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PRF1; PFP; Perforin 1 (Pore Forming Protein); P1; Cytolysin; Lymphocyte Pore Forming Protein; Lymphocyte Pore-Forming Protein; FLH2; Perforin; Perforin 1 (Preforming Protein); HPLH2; Perforin-1; PFN1
- Gene ID
- 5551
- UniProt ID
- P14222
- Long Name
- Perforin 1
- Cellular Localization
- Cell membrane; Endosome; Lysosome; Membrane; Secreted
- Post Translation Modifications
- Disulfide bond; Glycoprotein
- Protein Refseq
- NP_001076585.1
- Function
- Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed:20889983, PubMed:21037563, PubMed:24558045, PubMed:9058810, PubMed:9164947).; Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease (PubMed:9058810).; Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed:20889983, PubMed:21037563).; Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed:20038786, PubMed:20225066, PubMed:24558045, PubMed:32299851).; Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (PubMed:24558045).; Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "PRF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1853z | Mouse Anti-PRF1 Recombinant Antibody (clone 40G10) | FC, ICC, IF, IHC, IP | Mouse IgG2b |
| MOB-0981MZ | Recombinant Mouse Anti-Human PRF1 Antibody (clone 6C20) | IHC-P | Mouse antibody |
| VS3-CJ652 | Mouse Anti-PRF1 Recombinant Antibody (VS3-CJ652) | IHC | Mouse IgG |
| VS7-0425-WR681 | Mouse Anti-PRF1 Recombinant Antibody (clone 8A8C10) | WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5666 | Anti-PRF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5667 | Anti-Mouse PRF1 Immunohistochemistry Kit | IHC |
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