Mouse Anti-PRF1 Recombinant Antibody (clone 6F8C2) (CAT#: VS7-0425-WR680)

• Immunogen
• Recombinant human PRF1 (AA: extra 148-372) fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human PRF1

• Species Reactivity
• Human

• Clone
• 6F8C2

• Molecule Class
• Pore-Forming Protein

• Applications
• FC

• Conjugate
• Unconjugated

• Related Disease
• Hemophagocytic lymphohistiocytosis, familial, 2 (FHL2)

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• 0.05% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Flow Cytometry: 1:200 - 1:400
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• PRF1; PFP; Perforin 1 (Pore Forming Protein); P1; Cytolysin; Lymphocyte Pore Forming Protein; Lymphocyte Pore-Forming Protein; FLH2; Perforin; Perforin 1 (Preforming Protein); HPLH2; Perforin-1; PFN1

• Gene ID
• 5551

• UniProt ID
• P14222

• Long Name
• Perforin 1

• Cellular Localization
• Cell membrane; Endosome; Lysosome; Membrane; Secreted

• Post Translation Modifications
• Disulfide bond; Glycoprotein

• Protein Refseq
• NP_001076585.1

• Function
• Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed:20889983, PubMed:21037563, PubMed:24558045, PubMed:9058810, PubMed:9164947).; Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease (PubMed:9058810).; Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed:20889983, PubMed:21037563).; Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed:20038786, PubMed:20225066, PubMed:24558045, PubMed:32299851).; Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (PubMed:24558045).; Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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