Mouse Anti-PRF1 Recombinant Antibody (VS3-CJ652) (CAT#: VS3-CJ652)
This product is a mouse antibody that recognizes human PRF1.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37940/if_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37940/if_selected.jpg
Figure 2 IHC staining of human spleen
(Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2436/ihc_selected.jpg
(Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2436/ihc_selected.jpg
Figure 3 Spleen
(Cells in red pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2436/6381_B_8_4.jpg
(Cells in red pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2436/6381_B_8_4.jpg
Figure 4 RNA cell line category: Cell line enriched (HDLM-2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180644-PRF1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180644-PRF1/subcellular
Specifications
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence within human Perforin 1
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PRF1
- Species Reactivity
- Human
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.2% BSA, PBS.
- Preservative
- 0.01% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry (1:100-1:300).
Target
- Alternative Names
- P1; PFP; HPLH2
- Gene ID
- 5551
- UniProt ID
- P14222
- Sequence Similarities
- Belongs to the complement C6/C7/C8/C9 family.
- Cellular Localization
- Cell membrane, Endosome, Lysosome, Membrane, Secreted
- Post Translation Modifications
- N-glycosylated.
- Protein Refseq
- NP_001076585.1; NP_005032.2
- Function
- Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed:9058810, PubMed:9164947, PubMed:20889983, PubMed:21037563).
Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease (PubMed:9058810).
Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes (PubMed:20889983, PubMed:21037563).
Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes (PubMed:20038786, PubMed:20225066, PubMed:32299851).
Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1853z | Mouse Anti-PRF1 Recombinant Antibody (clone 40G10) | FC, ICC, IF, IHC, IP | Mouse IgG2b |
| MOB-0981MZ | Recombinant Mouse Anti-Human PRF1 Antibody (clone 6C20) | IHC-P | Mouse antibody |
| VS7-0425-WR680 | Mouse Anti-PRF1 Recombinant Antibody (clone 6F8C2) | FC | Mouse IgG1 |
| VS7-0425-WR681 | Mouse Anti-PRF1 Recombinant Antibody (clone 8A8C10) | WB, IHC, FC | Mouse IgG1 |
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