Mouse Anti-PRF1 Recombinant Antibody (VS3-CJ652) (CAT#: VS3-CJ652)
This product is a mouse antibody that recognizes human PRF1.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37940/if_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37940/if_selected.jpg
Figure 2 IHC staining of human spleen
(Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2436/ihc_selected.jpg
(Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2436/ihc_selected.jpg
Figure 3 Spleen
(Cells in red pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2436/6381_B_8_4.jpg
(Cells in red pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2436/6381_B_8_4.jpg
Figure 4 RNA cell line category: Cell line enriched (HDLM-2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180644-PRF1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180644-PRF1/subcellular
Specifications
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence within human Perforin 1
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PRF1
- Species Reactivity
- Human
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.2% BSA, PBS.
- Preservative
- 0.01% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry (1:100-1:300).
Target
- Alternative Names
- P1; PFP; HPLH2
- Gene ID
- 5551
- UniProt ID
- P14222
- Sequence Similarities
- Belongs to the complement C6/C7/C8/C9 family.
- Cellular Localization
- Cell membrane, Endosome, Lysosome, Membrane, Secreted
- Post Translation Modifications
- N-glycosylated.
- Protein Refseq
- NP_001076585.1; NP_005032.2
- Function
- Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed:9058810, PubMed:9164947, PubMed:20889983, PubMed:21037563).
Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease (PubMed:9058810).
Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes (PubMed:20889983, PubMed:21037563).
Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes (PubMed:20038786, PubMed:20225066, PubMed:32299851).
Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PRF1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1853z | Mouse Anti-PRF1 Recombinant Antibody (clone 40G10) | FC, ICC, IF, IHC, IP | Mouse IgG2b |
| MOB-0981MZ | Recombinant Mouse Anti-Human PRF1 Antibody (clone 6C20) | IHC-P | Mouse antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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