Rabbit Anti-PRKAR1A Recombinant Antibody (clone 1C3) (CAT#: ZG-0521U)
Rabbit Anti-PRKAR1A Antibody (clone 1C3) displayed high specificity against the PRKAR1A protein of Human.
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WB
Figure 1 Rabbit Anti-PRKAR1A Antibody (ZG-0521U) in WB
Western Blot
Positive WB detected in: U87 whole cell lysate
All lanes: PRKAR1A antibody at 1.1μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43, 39 KDa
Observed band size: 43 KDa
IF
Figure 2 Rabbit Anti-PRKAR1A Antibody (ZG-0521U) in IF
Immunofluorescence staining of Hela cells with ZG-0521U at 1:36, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human PRKAR1A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PRKAR1A
- Species Reactivity
- Human
- Clone
- 1C3
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Myxoma, Intracardiac and Carney Complex, Type 1.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- cAMP-dependent protein kinase type I-alpha regulatory subunit; Tissue-specific extinguisher 1; TSE1; PRKAR1A; PKR1; PRKAR1; TSE1
- Gene ID
- 5573
- UniProt ID
- P10644
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
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| MOB-2272z | Mouse Anti-PRKAR1A Recombinant Antibody (clone 18F5) | WB, ICC, IF, IHC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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