Rabbit Anti-PRKAR1A Recombinant Antibody (clone 1C3) (CAT#: ZG-0521U)
Rabbit Anti-PRKAR1A Antibody (clone 1C3) displayed high specificity against the PRKAR1A protein of Human.
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WB
Figure 1 Rabbit Anti-PRKAR1A Antibody (ZG-0521U) in WB
Western Blot
Positive WB detected in: U87 whole cell lysate
All lanes: PRKAR1A antibody at 1.1μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43, 39 KDa
Observed band size: 43 KDa
IF
Figure 2 Rabbit Anti-PRKAR1A Antibody (ZG-0521U) in IF
Immunofluorescence staining of Hela cells with ZG-0521U at 1:36, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human PRKAR1A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PRKAR1A
- Species Reactivity
- Human
- Clone
- 1C3
- Applications
- ELISA, WB, IF
- Conjugate
- Unconjugated
- Related Disease
- Myxoma, Intracardiac and Carney Complex, Type 1.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:20-1:200
Target
- Alternative Names
- cAMP-dependent protein kinase type I-alpha regulatory subunit; Tissue-specific extinguisher 1; TSE1; PRKAR1A; PKR1; PRKAR1; TSE1
- Gene ID
- 5573
- UniProt ID
- P10644
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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For Research Use Only. Not For Clinical Use.
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