Rabbit Anti-PRKCD (pS299) Recombinant Antibody (clone R06-4A5) (CAT#: VS3-WK2038)

Name: Rabbit Anti-PRKCD (pS299) Recombinant Antibody (clone R06-4A5)
SKU: VS3-WK2038
Rating: 5 (1 reviews)

This product is a rabbit recombinant antibody that is specific to phosphorylated PRKCD. The target of this antibody is phospho-PRKCD (Ser299). The clone R06-4A5 can be used in WB analysis at a dilution of 1/500-1/1000, IP analysis at a dilution of 1/20, and IHC staining at a dilution of 1/50-1/100. The molecular weight of PRKCD (pSer299) observed in WB experiments using this antibody is 78 kDa.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Rabbit Anti-PRKCD (pSer299) Recombinant Antibody (clone R06-4A5) in WB.

Figure 1 Rabbit Anti-PRKCD (pSer299) Recombinant Antibody (clone R06-4A5) in WB.

Western blot analysis of phospho-PKC delta (Ser299) in A549, HL-60 lysates using anti-PRKCD (pSer299) antibody, Cat# VS3-WK2038.

Subcellular Location

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1863/if_selected.jpg

Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1890/if_selected.jpg

Figure 3 Cerebral cortex
(Glial cells
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous

Neuropil
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_B_8_5.jpg

Normal Tissue

Figure 4 Colon
(Glandular cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_A_8_3.jpg

Figure 5 Gallbladder
(Glandular cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_A_6_4.jpg

Figure 6 Kidney
(Cells in glomeruli
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_A_9_5.jpg

Figure 7 Testis
(Sertoli cells
Staining:

Medium

Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_A_6_6.jpg

Figure 8 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:

High

Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_A_9_8.jpg

RNA Expression

Figure 9 RNA cell line category: Cell line enhanced (SK-BR-3, THP-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163932-PRKCD

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthetic phosphopeptide corresponding to residues Ser299 of human PKC delta

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Phospho-Ser299 of human PRKCD

Species Reactivity
Human

Clone
R06-4A5

Applications
WB, IHC-P, IP

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purification
Protein A affinity purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
WB: 1/500-1/1000
IHC: 1/50-1/100
IP: 1/20

Target

Alternative Names
MAY1; PKCD; ALPS3; CVID9; nPKC-delta

Gene ID
5580

UniProt ID
Q05655

Long Name
Protein kinase C delta

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "PRKCD"

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IgG Antibody Products Rabbit Monoclonal Antibody Products Antibody Magnetic Beads IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry

CAT	Product Name	Application	Type
MOB-1323z	Mouse Anti-PRKCD Recombinant Antibody (clone 33C7)	WB, IP, IF, IHC, ELISA	Mouse IgG1, κ
MOB-0774MZ	Recombinant Mouse Anti-Human PRKCD Antibody	WB	Mouse antibody
ZG-0337U	Rabbit Anti-PRKCD Recombinant Antibody (clone K15-K)	ELISA, WB, IP	Rabbit IgG
ZG-0416U	Rabbit Anti-PRKCD Recombinant Antibody (clone R20-F), FITC	FC	Rabbit IgG
VS3-WK880	Rabbit Anti-PRKCD Recombinant Antibody (VS3-WK880)	WB, ICC, IF, IHC	Rabbit IgG

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