Rabbit Anti-PRKCD (pS299) Recombinant Antibody (clone R06-4A5)
CAT#: VS3-WK2038
This product is a rabbit recombinant antibody that is specific to phosphorylated PRKCD. The target of this antibody is phospho-PRKCD (Ser299). The clone R06-4A5 can be used in WB analysis at a dilution of 1/500-1/1000, IP analysis at a dilution of 1/20, and IHC staining at a dilution of 1/50-1/100. The molecular weight of PRKCD (pSer299) observed in WB experiments using this antibody is 78 kDa.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1863/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1890/if_selected.jpg
Subcellular Location
Figure 3 Cerebral cortex
Glial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_A_8_3.jpg
Normal Tissue
Figure 5 Gallbladder
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_A_6_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13225/34383_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (SK-BR-3, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163932-PRKCD
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WB
Figure 1 Rabbit Anti-PRKCD (pSer299) Recombinant Antibody (clone R06-4A5) in WB.
Western blot analysis of phospho-PKC delta (Ser299) in A549, HL-60 lysates using anti-PRKCD (pSer299) antibody, Cat# VS3-WK2038.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Ser299 of human PKC delta
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser299 of human PRKCD
- Species Reactivity
- Human
- Clone
- R06-4A5
- Applications
- WB, IHC-P, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IP: 1/20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
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