Mouse Anti-PRMT6 Recombinant Antibody (VS3-FY2844) (CAT#: VS3-FY2844)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human and Monkey PRMT6

• Species Reactivity
• Human, Monkey

• Applications
• Western Blot, Immunohistochemistry, Immunocytochemistry

• Conjugate
• Unconjugated, FITC

• Related Disease
• Male Infertility and Azoospermia

• Clonality
• Monoclonal Antibody

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100
  Immunocytochemistry: 1/50-1/200

• Alternative Names
• HRMT1L6

• Gene ID
• 55170

• UniProt ID
• Q96LA8

• Sequence Similarities
• Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT6 subfamily.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Automethylation enhances its stability and antiretroviral activity.

• Protein Refseq
• NP_060607.2

• Function
• Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA (PubMed:17898714, PubMed:18077460, PubMed:18079182, PubMed:19405910, PubMed:30420520).
  Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates (PubMed:17898714, PubMed:18077460, PubMed:18079182, PubMed:19405910).
  Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a (PubMed:17898714, PubMed:18079182, PubMed:18077460).
  H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3) (PubMed:17898714, PubMed:18077460).
  Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53 (PubMed:19509293).
  Repression of TP53 blocks cellular senescence (By similarity).
  Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity (PubMed:16600869).
  Methylates HMGA1 (PubMed:16157300, PubMed:16159886).
  Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator (By similarity).
  May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC) (PubMed:17267505).
  Methylates GPS2, protecting GPS2 from ubiquitination and degradation (By similarity).
  Methylates SIRT7, inhibiting SIRT7 histone deacetylase activity and promoting mitochondria biogenesis (PubMed:30420520).

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

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