Anti-PTPRB Antibody Prodrug, Protease Activated (VS-1025-YC187)
CAT#: VS-1025-YC187
The Anti-PTPRB Antibody Prodrug, Protease Activated (VS-1025-YC187) is an antibody prodrug comprised of a recombinant rabbit antibody targeting human PTPRB, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to PTPRB protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to PTPRB effectively. PTPRB (VE-PTP) is a tyrosine phosphatase expressed on endothelial cells that is crucial for maintaining vascular integrity. While not an oncogene, it is vital in the tumor microenvironment for regulating angiogenesis and blood vessel stability, influencing metastatic efficiency.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67868/1717_A6_22_cr57f514fc87452_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4782/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to cell junctions.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67868/1315_H7_2_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67868/1287_H7_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4782/11835_B_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4782/11671_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4782/11671_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (EFO-21, hTERT-RPE1, HUVEC TERT2, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000127329-PTPRB
❮
❯
Specifications
- Host Animal
- Rabbit
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human PTPRB
- Target
- PTPRB
- Species Reactivity
- Human
- Isotype
- IgG
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- This antibody is expected to recognize both reported isoforms of protein tyrosine phosphatase, receptor type, beta polypeptide (PTPRB), which is critical in physiological and pathological events such as cell migration, adhesion and transformation.
- Alternative Names
- PTPRB; PTPB; HPTPB; VEPTP; HPTP-BETA; R-PTP-BETA
- Gene ID
- 5787
- UniProt ID
- P23467
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3104z | Mouse Anti-PTPRB Recombinant Antibody (clone 7C10) | ICC, IF | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-292CT | Human Anti-PTPRB Recombinant Antibody (TAB-292CT) | ELISA, FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-292CT-S(P) | Human Anti-PTPRB Recombinant Antibody; scFv Fragment (TAB-292CT-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-292CT-F(E) | Human Anti-PTPRB Recombinant Antibody; Fab Fragment (TAB-292CT-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1296-CN | Recombinant Mouse Anti-PTPRB Monoclonal Antibody (2-A2) | IF, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS1-YJ199 | Mouse Anti-Ptprb Recombinant Antibody (clone 122.2) | IF, ICC, WB, IP | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC185 | Recombinant Anti-PTPRB Vesicular Antibody, EV Displayed (VS-0425-YC185) | ELISA, FC, Cell-uptake |
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