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Anti-PUM1 Immunohistochemistry Kit (CAT#: VS-0325-XY1831)

The PUM1 IHC Kit provides a comprehensive set of reagents, ensuring a seamless experimental process. Its user - friendly nature allows for straightforward operation. Whether working with paraffin - embedded tissues or frozen sections, this kit can effectively detect PUM1. This is important for studies on post - transcriptional regulation and the role of PUM1 in binding to specific mRNA sequences and influencing their stability and translation efficiency.

Specific Inquiry
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27424/583_E9_1_selected.jpg
Figure 2 IHC staining of human colon
Figure 2 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27424/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27424/57187_B_9_5.jpg
Figure 4 Stomach
Figure 4 Stomach
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27424/56911_A_4_1.jpg
Figure 5 Smooth muscle
Figure 5 Smooth muscle
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27424/56911_A_8_3.jpg
Figure 6 Liver
Figure 6 Liver
(Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27424/56911_A_9_4.jpg
Figure 7 Kidney
Figure 7 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27424/56911_A_8_5.jpg
Figure 8 Testis
Figure 8 Testis
(Cells in seminiferous ducts
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27424/56911_A_4_6.jpg
Figure 9 Lymph node
Figure 9 Lymph node
(Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27424/56911_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134644-PUM1/subcellular

Specifications

  • Application
  • IHC
  • Size
  • 50 Tests
  • Species Reactivity
  • Human, Mouse, Rat
  • Target
  • PUM1
  • Primary Antibody
  • Rabbit Anti-PUM1 Antibody
  • Secondary Antibody
  • Goat anti-Rabbit Antibody, HRP
  • Sample Type
  • FFPE tissue; Frozen section tissue
  • Kit Storage
  • All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.

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CAT Product Name Application Type
MOR-2941 Hi-Affi™ Recombinant Rabbit Anti-PUM1 Monoclonal Antibody (DS2941AB) FC, ICC/IF, IHC-P, IP, WB IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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