Mouse Anti-Rab3a Recombinant Antibody (clone 6D1A12) (CAT#: VS7-0425-WR741)

• Immunogen
• Recombinant human Rab3a (AA: 1-220) fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human Rab3a

• Species Reactivity
• Human

• Clone
• 6D1A12

• Molecule Class
• Small GTPases

• Applications
• FC

• Conjugate
• Unconjugated

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• 0.05% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Flow Cytometry: 1:200 - 1:400
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• RAB3A, member RAS oncogene family

• Gene ID
• 5864

• UniProt ID
• P20336

• Long Name
• RAB3A, member RAS oncogene family

• Cellular Localization
• Cell membrane; Cell projection; Cytoplasm; Cytoplasmic vesicle; Lysosome; Membrane; Synapse

• Post Translation Modifications
• Lipoprotein; Methylation; Phosphoprotein; Prenylation

• Protein Refseq
• NP_002857.1

• Function
• Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane (By similarity).; Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane (By similarity).; Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed (By similarity).; Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells (By similarity).; Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9 (PubMed:27325790).; Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1 (PubMed:22248876, PubMed:30599141).; Also plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT (By similarity).; Interacts with MADD (via uDENN domain); the GTP-bound form is preferred for interaction (By similarity).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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