AbPlus™ Anti-RAC1 Magnetic Beads (JF10-11) (CAT#: VS-0424-XY221)
The AbPlus Anti-RAC1 Magnetic Beads (JF10-11) is an innovative affinity resin which is bound with anti-RAC1 specific antibody (JF10-11). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of RAC1 protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35994/265_F2_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35994/265_F2_2_selected.jpg

Figure 2 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35994/ihc_selected.jpg
(Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/35994/ihc_selected.jpg

Figure 3 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47820/112664_A_8_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47820/112664_A_8_5.jpg

Figure 4 Testis
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47820/112664_A_5_6.jpg
(Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47820/112664_A_5_6.jpg

Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47820/112664_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47820/112664_A_9_3.jpg

Figure 6 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47820/112664_A_9_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47820/112664_A_9_8.jpg

Figure 7 Skin
(Keratinocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47820/112664_B_9_1.jpg
(Keratinocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47820/112664_B_9_1.jpg

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136238-RAC1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136238-RAC1
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magrose bead (> 50 μmol/mL gel)
- Bead Ligand
- Anti-RAC1 specific antibody (JF10-11)
- Target
- RAC1
- Immunogen
- Recombinant protein
- Target Species
- Human, Mouse, Rat, Zebrafish
- Antibody Clone
- JF10-11
- Bead Capacity
- 20-30 mg/mL binding antibody
- Bead size
- 10-37 μm
- Stability
- pH 2-14
- Format
- 20% Suspension
- Buffer
- PBS, pH 7.4, with 1% BSA
- Preservative
- 0.03% Proclin 300
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.
- Alternative Names
- MIG5; MRD48; Rac-1; TC-25; p21-Rac1
- Gene ID
- 5879
- UniProt ID
- P63000
Related Resources
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2124z | Mouse Anti-RAC1 Recombinant Antibody (clone 35A6) | WB, IHC, IP | Mouse IgG2b |
VS3-QX948 | Mouse Anti-RAC1 Recombinant Antibody (clone 1301CT276.121.104) | WB, IHC, FC | Mouse IgG2b |
VS3-WK421 | Rabbit Anti-RAC1 Recombinant Antibody (clone JF10-11) | WB, ICC, IF, IP | Rabbit IgG |
VS3-WK561 | Rabbit Anti-RAC1 Recombinant Antibody (clone JM11-29) | WB, ICC, IF, IHC | Rabbit IgG |
VS3-FY2857 | Mouse Anti-RAC1 Recombinant Antibody (VS3-FY2857) | WB, IHC, FC | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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