Mouse Anti-RAC1 Recombinant Antibody (VS3-FY2857)
CAT#: VS3-FY2857
The anti-RAC1 antibody is a mouse recombinant monoclonal antibody that is specifically created to bind human, mouse, rat RAC1 protein. The calculated molecular weight of the protein is about 21.5 kDa. This monoclonal antibody proves valuable in disease research related to Intellectual Developmental Disorder, Autosomal Dominant 48 and Microcephaly-Corpus Callosum And Cerebellar Vermis Hypoplasia-Facial Dysmorphism-Intellectual Disability Syndrom. This monoclonal antibody is suitable for employment in Western Blot (1/500-1/1000), Immunohistochemistry (1/50-1/200), Flow cytometry (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35994/265_F2_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35994/ihc_selected.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47820/112664_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47820/112664_A_5_6.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47820/112664_A_9_3.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47820/112664_A_9_8.jpg
Normal Tissue
Figure 7 Skin
Keratinocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47820/112664_B_9_1.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136238-RAC1
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse and Rat RAC1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot, Immunohistochemistry, Flow cytometry
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 21.5 kDa.
- Related Disease
- Intellectual Developmental Disorder, Autosomal Dominant 48 and Microcephaly-Corpus Callosum And Cerebellar Vermis Hypoplasia-Facial Dysmorphism-Intellectual Disability Syndrom
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/50-1/100
Target
- Alternative Names
- Rac Family Small GTPase 1; Ras-Related C3 Botulinum Toxin Substrate 1 (Rho Family, Small GTP Binding Protein Rac1); Cell Migration-Inducing Gene 5 Protein; Ras-Like Protein TC25; P21-Rac1; Ras-Related C3 Botulinum Toxin Substrate 1
- Gene ID
- 5879
- UniProt ID
- P63000
- Sequence Similarities
- Belongs to the small GTPase superfamily. Rho family.
- Cellular Localization
- Cell membrane
Lipid-anchor
Cytoplasmic side
Melanosome
Cytoplasm
Cell projection, lamellipodium
Cell projection, dendrite
- Post Translation Modifications
- GTP-bound active form is ubiquitinated by HACE1, leading to its degradation by the proteasome. (P63000-RAC1_HUMAN)
Phosphorylated by AKT at Ser-71. (P63000-RAC1_HUMAN)
Ubiquitinated at Lys-166 in a FBXL19-mediated manner; leading to proteasomal degradation. (P63000-RAC1_HUMAN)
(Microbial infection) AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively
- Protein Refseq
- NP_061485.1
- Function
- Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states.
In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles.
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Related Signaling Pathways
Canonical Wnt Signaling Pathway
MAPK Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "RAC1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2124z | Mouse Anti-RAC1 Recombinant Antibody (clone 35A6) | WB, IHC, IP | Mouse IgG2b |
| VS3-QX948 | Mouse Anti-RAC1 Recombinant Antibody (clone 1301CT276.121.104) | WB, IHC, FC | Mouse IgG2b |
| VS3-WK421 | Rabbit Anti-RAC1 Recombinant Antibody (clone JF10-11) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-WK561 | Rabbit Anti-RAC1 Recombinant Antibody (clone JM11-29) | WB, ICC, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0486MZ | Chicken Anti-RAC1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2970 | Hi-Affi™ Recombinant Rabbit Anti-RAC1 Monoclonal Antibody (DS2970AB) | ELISA, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY221 | AbPlus™ Anti-RAC1 Magnetic Beads (JF10-11) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1858 | Anti-RAC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5943 | Anti-Human RAC1 Immunohistochemistry Kit | IHC |
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