Mouse Anti-RAD18 Recombinant Antibody (clone 2G8D12) (CAT#: VS7-0425-WR754)
This product is a mouse IgG1 antibody that binds to human RAD18. The antibody 2G8D12 recognizes specific amino acid domain of human RAD18 (AA: 332-430). It can be used for FC assay.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunohistochemical staining of human lymph node shows distinct nuclear positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6716/ihc_selected.jpg

(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuropil Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8752/24838_B_7_5.jpg

(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8752/25120_A_8_3.jpg

(Collecting ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8752/25120_A_8_5.jpg

(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8752/25120_A_6_6.jpg

(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8752/25120_A_8_8.jpg

(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000070950-RAD18
Specifications
- Immunogen
- Recombinant human RAD18 (AA: 332-430) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human RAD18
- Species Reactivity
- Human
- Clone
- 2G8D12
- Molecule Class
- E3 Ubiquitin Ligase
- Applications
- FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RAD18; E3 Ubiquitin Protein Ligase; RING-Type E3 Ubiquitin Transferase RAD18; Postreplication Repair Protein RAD18; RING Finger Protein 73; HRAD18; RNF73; HHR18
- Gene ID
- 56852
- UniProt ID
- Q9NS91
- Long Name
- RAD18, E3 Ubiquitin Protein Ligase
- Cellular Localization
- Cytoplasm; Cytoskeleton; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_064550.3
- Function
- DNA-binding; Transferase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "RAD18"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-0808CT | Recombinant Mouse anti-Human RAD18 Monoclonal antibody (EML1638) | ICC/IF, IHC-P, WB | |
MRO-1313-CN | Recombinant Rabbit Anti-RAD18 Monoclonal Antibody (CBACN-474) | WB, IF, IHC, FC | Rabbit IgG |
VS3-WK1171 | Rabbit Anti-RAD18 Recombinant Antibody (VS3-WK1171) | WB, ICC, IF, IHC, FC | Rabbit IgG |
VS7-0425-WR755 | Mouse Anti-RAD18 Recombinant Antibody (clone 2G8C10) | FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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