Mouse Anti-RAD18 Recombinant Antibody (clone 2G8D12) (CAT#: VS7-0425-WR754)
This product is a mouse IgG1 antibody that binds to human RAD18. The antibody 2G8D12 recognizes specific amino acid domain of human RAD18 (AA: 332-430). It can be used for FC assay.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Normal Tissue
RNA Expression
Figure 1 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows distinct nuclear positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6716/ihc_selected.jpg
(Immunohistochemical staining of human lymph node shows distinct nuclear positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6716/ihc_selected.jpg
Figure 2 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuropil Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8752/24838_B_7_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuropil Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8752/24838_B_7_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8752/25120_A_8_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8752/25120_A_8_3.jpg
Figure 4 Kidney
(Collecting ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8752/25120_A_8_5.jpg
(Collecting ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8752/25120_A_8_5.jpg
Figure 5 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8752/25120_A_6_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8752/25120_A_6_6.jpg
Figure 6 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8752/25120_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8752/25120_A_8_8.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000070950-RAD18
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000070950-RAD18
Specifications
- Immunogen
- Recombinant human RAD18 (AA: 332-430) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human RAD18
- Species Reactivity
- Human
- Clone
- 2G8D12
- Molecule Class
- E3 Ubiquitin Ligase
- Applications
- FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RAD18; E3 Ubiquitin Protein Ligase; RING-Type E3 Ubiquitin Transferase RAD18; Postreplication Repair Protein RAD18; RING Finger Protein 73; HRAD18; RNF73; HHR18
- Gene ID
- 56852
- UniProt ID
- Q9NS91
- Long Name
- RAD18, E3 Ubiquitin Protein Ligase
- Cellular Localization
- Cytoplasm; Cytoskeleton; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_064550.3
- Function
- DNA-binding; Transferase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0808CT | Recombinant Mouse anti-Human RAD18 Monoclonal antibody (EML1638) | ICC/IF, IHC-P, WB | |
| MRO-1313-CN | Recombinant Rabbit Anti-RAD18 Monoclonal Antibody (CBACN-474) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK1171 | Rabbit Anti-RAD18 Recombinant Antibody (VS3-WK1171) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS7-0425-WR755 | Mouse Anti-RAD18 Recombinant Antibody (clone 2G8C10) | FC | Mouse IgG1 |
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