Mouse Anti-RAD23A Recombinant Antibody (clone 1E4D6)
CAT#: VS7-0425-WR757
This product is a mouse IgG2b antibody that binds to human RAD23A. The antibody 1E4D6 recognizes specific amino acid domain of human RAD23A (AA: 1-363). It can be used for WB and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26418/1745_D2_14_cr58060d5f066ef_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57003/992_B7_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human salivary gland
Immunohistochemical staining of human salivary gland shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65599/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65599/146053_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65599/146053_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65599/146053_A_8_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65599/146053_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65599/146053_A_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000179262-RAD23A
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Specifications
- Immunogen
- Recombinant human RAD23A (AA: 1-363) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human RAD23A
- Species Reactivity
- Human
- Clone
- 1E4D6
- Molecule Class
- Multiubiquitin Chain Receptor
- Applications
- WB, ELSIA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RAD23 Homolog A, Nucleotide Excision Repair Protein; RAD23, Yeast Homolog, A; HHR23A; HR23A; UV Excision Repair Protein RAD23 Homolog A; RAD23 (S. Cerevisiae) Homolog A; RAD23 Homolog A (S. Cerevisiae)
- Gene ID
- 5886
- UniProt ID
- P54725
- Long Name
- RAD23 Homolog A, Nucleotide Excision Repair Protein
- Cellular Localization
- Nucleus; Proteasome
- Post Translation Modifications
- Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001257291.1
- Function
- Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.; Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.; (Microbial infection) Involved in Vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 Vpr with the host proteasome.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0591CT | Recombinant Mouse anti-Human RAD23A Monoclonal antibody (EML700) | WB | |
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| VS7-0425-WR756 | Mouse Anti-RAD23A Recombinant Antibody (clone 3E3F4) | IHC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2976 | Hi-Affi™ Recombinant Rabbit Anti-RAD23A Monoclonal Antibody (DS2976AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
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| VS13-YC977 | CytoStream™ Rabbit Anti-RAD23A Recombinant Antibody (VS13-YC977) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-0525-XY5953 | Anti-RAD23A Immunohistochemistry Kit | IHC |
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