Rabbit Anti-RAD23A Recombinant Antibody (clone R06-7H6)
CAT#: VS3-FY2009
The R06-7H6 recombinant monoclonal antibody exhibits specific detecting to human RAD23A. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, IP. This hHR23A antibody has been rigorously validated and is widely used in western blot analysis of hHR23A in 3T3 lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26418/1745_D2_14_cr58060d5f066ef_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57003/992_B7_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human salivary gland
Immunohistochemical staining of human salivary gland shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65599/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65599/146053_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65599/146053_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65599/146053_A_8_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65599/146053_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65599/146053_A_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000179262-RAD23A
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WB
Figure 1 Anti-RAD23A Antibody (VS3-FY2009) in WB.
The hHR23A expression in 3T3 lysates were detected using the anti-hHR23A by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human hHR23A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse RAD23A
- Species Reactivity
- Human, Mouse
- Clone
- R06-7H6
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 40 kDa, while the observed molecular weight stands at 52 kDa.
- Related Disease
- Xeroderma Pigmentosum, Variant Type and Xeroderma Pigmentosum, Complementation Group C
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- RAD23 Homolog A, Nucleotide Excision Repair Protein; RAD23, Yeast Homolog, A; HHR23A; HR23A; UV Excision Repair Protein RAD23 Homolog A; RAD23 (S. Cerevisiae) Homolog A; RAD23 Homolog A (S. Cerevisiae)
- Gene ID
- 5886
- UniProt ID
- P54725
- Sequence Similarities
- Belongs to the RAD23 family.
- Cellular Localization
- Nucleus, Proteasome
- Post Translation Modifications
- Ubiquitination at Lys8, Lys16, Lys26, Lys53, Lys78, Lys80, and Lys122 (NX_P54725 [NX_P54725-1])
Modification sites at PhosphoSitePlus (P54725)
Glycosylation from GlyGen (P54725) 3 sites, 1 O-linked glycan (3 sites)
- Protein Refseq
- NP_001257291.1; NP_001257292.1; NP_005044.1
- Function
- Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RAD23A"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0591CT | Recombinant Mouse anti-Human RAD23A Monoclonal antibody (EML700) | WB | |
| VS3-QX949 | Mouse Anti-RAD23A Recombinant Antibody (clone 3E-4F) | WB, IHC, FC | Mouse IgG2b |
| VS7-0425-WR756 | Mouse Anti-RAD23A Recombinant Antibody (clone 3E3F4) | IHC, FC | Mouse IgG2b |
| VS7-0425-WR757 | Mouse Anti-RAD23A Recombinant Antibody (clone 1E4D6) | WB, ELSIA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2976 | Hi-Affi™ Recombinant Rabbit Anti-RAD23A Monoclonal Antibody (DS2976AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC977 | CytoStream™ Rabbit Anti-RAD23A Recombinant Antibody (VS13-YC977) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5953 | Anti-RAD23A Immunohistochemistry Kit | IHC |
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