Mouse Anti-RALA Recombinant Antibody (clone 4G8C7)
CAT#: VS7-0425-WR762
This product is a mouse IgG1 antibody that binds to human RALA. The antibody 4G8C7 recognizes specific amino acid domain of human RALA (AA: 71-203). It can be used for WB, IHC, ICC, and FC assays. It cross reacts with mouse and monkey.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47037/1201_H1_5_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65232/146930_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65232/146930_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65232/146930_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65232/146930_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65232/146930_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/65232/146930_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HUVEC TERT2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000006451-RALA
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Specifications
- Immunogen
- Recombinant human RALA (AA: 71-203) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human RALA
- Species Reactivity
- Human, Mouse, Monkey
- Clone
- 4G8C7
- Molecule Class
- Small GTPases
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Hiatt-Neu-Cooper neurodevelopmental syndrome (HINCONS)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RALA; Ras Related GTP Binding Protein A; Ras Family Small GTP Binding Protein RALA; V-Ral Simian Leukemia Viral Oncogene Homolog A (Ras Related); Ras-Related Protein Ral-A; RAS-Like Protein A
- Gene ID
- 5898
- UniProt ID
- P11233
- Long Name
- V-Ral Simian Leukemia Viral Oncogene Homolog A (Ras Related)
- Cellular Localization
- Cell membrane; Membrane; Mitochondrion
- Post Translation Modifications
- Glycoprotein; Lipoprotein; Methylation; Phosphoprotein; Prenylation
- Protein Refseq
- NP_005393.2
- Function
- Hydrolase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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