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  • Rabbit Anti-RANBP3 Recombinant Antibody (clone R97-2F-2)

Rabbit Anti-RANBP3 Recombinant Antibody (clone R97-2F-2) (CAT#: VS3-FY2134)

Clone R97-2F-2 is a recombinant monoclonal antibody that exhibits a high affinity for human RANBP3 protein, a calculated weight of 60 kDa. It cross-reacts with mouse. The recommend antibody dilution is determined as western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20), flow cytometry (dilution 1/50).


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  • Gene Expression
  • Datasheet
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  • COA
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43375/if_selected.jpg
Figure 2 IF staining of human cell line A-431
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43389/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43375/98690_B_7_5.jpg
Figure 4 Colon
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43375/98690_A_7_3.jpg
Figure 5 Liver
Figure 5 Liver
(Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43375/98690_A_8_4.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43375/98690_A_9_5.jpg
Figure 7 Testis
Figure 7 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43375/98690_A_6_6.jpg
Figure 8 Lymph node
Figure 8 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43375/98690_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000031823-RANBP3

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human and Mouse RANBP3
  • Species Reactivity
  • Human, Mouse
  • Clone
  • R97-2F-2
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation, Flow Cytometry
  • Conjugate
  • Unconjugated
  • MW
  • The calculated weight of the target protein is approximately 60 kDa, while the observed molecular weight stands at 90kDa.

Product Property

  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl, 50% glycerol
  • Preservative
  • 0.02% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/100-1/200
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/20
    Flow Cytometry: 1/50

Target

  • Alternative Names
  • RAN Binding Protein 3; RanBP3
  • Cellular Localization
  • Cytoplasm, Nucleus
  • Post Translation Modifications
  • Ubiquitination at Lys413 (NX_Q9H6Z4 [NX_Q9H6Z4-1])
    Modification sites at PhosphoSitePlus (Q9H6Z4)
    Glycosylation from GlyGen (Q9H6Z4) 1 site, 1 O-linked glycan (1 site)
  • Function
  • Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner.

Recommended Products

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "RANBP3"

Recombinant Antibody

CAT Product Name Application Type
MOB-1771CT Recombinant Mouse anti-Human RANBP3 Monoclonal antibody (EML784) WB

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2994 Hi-Affi™ Recombinant Rabbit Anti-RANBP3 Monoclonal Antibody (DS2994AB) WB, IHC, ICC, IP, FC IgG
MOR-4725 Hi-Affi™ Recombinant Rabbit Anti-RANBP3 Monoclonal Antibody (TH239DS) WB, IF, ICC IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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