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    Rabbit Anti-RANBP3 Recombinant Antibody (clone R97-2F-2) (CAT#: VS3-FY2134)

    Clone R97-2F-2 is a recombinant monoclonal antibody that exhibits a high affinity for human RANBP3 protein, a calculated weight of 60 kDa. It cross-reacts with mouse. The recommend antibody dilution is determined as western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20), flow cytometry (dilution 1/50).

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    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43375/if_selected.jpg
    Figure 2 IF staining of human cell line A-431
    Figure 2 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43389/if_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43375/98690_B_7_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43375/98690_A_7_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43375/98690_A_8_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43375/98690_A_9_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43375/98690_A_6_6.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/43375/98690_A_9_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000031823-RANBP3

    Specifications

    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human and Mouse RANBP3
    • Species Reactivity
    • Human, Mouse
    • Clone
    • R97-2F-2
    • Applications
    • Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation, Flow Cytometry
    • Conjugate
    • Unconjugated
    • MW
    • The calculated weight of the target protein is approximately 60 kDa, while the observed molecular weight stands at 90kDa.

    Product Property

    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS (pH 7.4), 150 mM NaCl, 50% glycerol
    • Preservative
    • 0.02% Sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Western Blot: 1/500-1/1000
      Immunohistochemistry: 1/100-1/200
      Immunofluorescence: 1/50-1/200
      Immunoprecipitation: 1/20
      Flow Cytometry: 1/50

    Target

    • Alternative Names
    • RAN Binding Protein 3; RanBP3
    • Cellular Localization
    • Cytoplasm, Nucleus
    • Post Translation Modifications
    • Ubiquitination at Lys413 (NX_Q9H6Z4 [NX_Q9H6Z4-1])
      Modification sites at PhosphoSitePlus (Q9H6Z4)
      Glycosylation from GlyGen (Q9H6Z4) 1 site, 1 O-linked glycan (1 site)
    • Function
    • Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner.

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    Please select product type
    IgG Antibodies Rabbit Monoclonal Antibodies IHC Kits & Antibodies
    CAT Product Name Application Type
    MOB-1771CT Recombinant Mouse anti-Human RANBP3 Monoclonal antibody (EML784) WB

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