Mouse Anti-RANBP9 Recombinant Antibody (clone 6E10H5)
CAT#: VS7-0425-WR766
This product is a mouse IgG1 antibody that binds to human RANBP9. The antibody 6E10H5 recognizes specific amino acid domain of human RANBP9 (AA: 453-680). It can be used for WB, IHC, and FC assays. It cross reacts with mouse.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line EFO-21
Immunofluorescent staining of human cell line EFO-21 shows localization to nucleoplasm, nuclear bodies & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76784/1937_H11_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/33767/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows distinct cytoplasmic and nuclear positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/33767/ihc_selected.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50007/123722_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50007/123722_A_9_4.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50007/123722_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000010017-RANBP9
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Specifications
- Immunogen
- Recombinant human RANBP9 (AA: 453-680) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human RANBP9
- Species Reactivity
- Human, Mouse
- Clone
- 6E10H5
- Molecule Class
- Small GTP Binding Protein
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RAN Binding Protein 9; Ran Binding Protein In The Microtubule Organizing Center; Ran-Binding Protein M; RANBP7; RANBPM; BPM-L
- Gene ID
- 10048
- UniProt ID
- Q96S59
- Long Name
- RAN Binding Protein 9
- Cellular Localization
- Cell membrane; Cytoplasm; Membrane; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_005484.2
- Function
- May act as scaffolding protein, and as adapter protein to couple membrane receptors to intracellular signaling pathways (Probable). Acts as a mediator of cell spreading and actin cytoskeleton rearrangement (PubMed:18710924).; Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (PubMed:29911972).; May be involved in signaling of ITGB2/LFA-1 and other integrins (PubMed:14722085).; Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway (PubMed:12147692).; Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen-induced transactivation (PubMed:12361945, PubMed:18222118).; Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity (PubMed:15558019).; Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
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- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
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Recommended Dilution Buffer
- CAT
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| MOR-2995 | Hi-Affi™ Recombinant Rabbit Anti-RANBP9 Monoclonal Antibody (DS2995AB) | WB, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
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| ZG-058R | Mouse Anti-RANBP9 Recombinant Antibody (ZG-058R) | WB, ICC | Mouse IgG |
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