Rabbit Anti-RASEF Recombinant Antibody (clone R03-1G6) (CAT#: VS3-FY1865)
The R03-1G6 recombinant monoclonal antibody exhibits a high affinity for human RASEF protein, a calculated weight of 83 kDa. It cross-reacts with mouse.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R03-1G6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunofluorescence (dilution range 1:50-1:200).
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21431/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21431/if_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/78596/167039_A_5_1_selected.jpg
(Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/78596/167039_A_5_1_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/78596/167039_B_8_5.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/78596/167039_B_8_5.jpg
Figure 4 Thyroid gland
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/78596/167039_B_1_5.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/78596/167039_B_1_5.jpg
Figure 5 Rectum
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/78596/167039_A_7_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/78596/167039_A_7_3.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/78596/167039_A_7_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/78596/167039_A_7_5.jpg
Figure 7 Testis
(Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/78596/167039_A_6_6.jpg
(Peritubular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/78596/167039_A_6_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (OE19, RT4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165105-RASEF/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165105-RASEF/subcellular
Specifications
- Immunogen
- Synthetic peptide of human RAB45
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse RASEF
- Species Reactivity
- Human, Mouse
- Clone
- R03-1G6
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 83 kDa, while the observed molecular weight stands at 83 kDa.
- Related Disease
- Cutaneous Adenocystic Carcinoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- TSG; RAB45
- Gene ID
- 158158
- UniProt ID
- Q8IZ41
- Sequence Similarities
- Belongs to the small GTPase superfamily. Rab family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Ubiquitination at Lys171, Lys366, and Lys388 (NX_Q8IZ41 [NX_Q8IZ41-1])
Modification sites at PhosphoSitePlus (Q8IZ41)
- Protein Refseq
- XP_005251787.1; XP_005251788.1; XP_047278782.1; XP_047278783.1; XP_054218043.1
- Function
- Binds predominantly GDP, and also GTP.
Acts as a dynein adapter protein that activates dynein-mediated transport and dynein-dynactin motility on microtubules.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1348CT | Recombinant Mouse anti-Human RASEF Monoclonal antibody (EML2132) | IHC-P, WB |
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