Goat Anti-Rat IgG-Fc Fragment Secondary antibody
CAT#: 2RD-A1490-YJ
This product is a Goat secondary antibody that recognizes Rat IgG-Fc Fragment.
Specifications
- Host Species
- Goat
- Type
- Goat IgG
- Specificity
- Rat IgG-Fc Fragment
- Species Reactivity
- Rat
- Applications
- WB, IHC, ICC, ELISA
- Conjugate
- Unconjugated, FITC, HRP, AP, Biotin are available
Product Property
- Concentration
- Please refer to the Certificate of Analysis for the lot-specific concentration.
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody can be used in Western Blot, Immunohistochemistry, Immunocytochemistry, Enzyme-linked Immunosorbent Assay. Optimal working dilutions should be determined experimentally by the investigator.
Target
- Alternative Names
- IgG-Fc Fragment; IgG-Fc; IgG
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Excellent Secondary Antibody
We used this polyclonal antibody as a secondary reagent to detect rat primary antibodies in our immunohistochemistry protocol. It specifically targets the Fc fragment, which helped reduce background binding that we sometimes see with whole IgG secondaries. The signal amplification was great, resulting in crisp staining of our tissue samples. A reliable and cost-effective secondary antibody for everyday lab use.
Specific Fc Recognition
In our sandwich ELISA development, we needed an antibody that would capture Rat IgG via the Fc region without interfering with antigen binding at the Fab end. This polyclonal antibody worked perfectly for that purpose. It showed strong binding capacity and good specificity. It has become a standard reagent in our assay development toolkit for rat model research.
Q&As
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We are looking for a secondary antibody. Is this product monoclonal or polyclonal, and what does it target?
A: This product is a Polyclonal Antibody. It is designed to target the Fc fragment of Rat IgG. Being polyclonal, it can bind to multiple epitopes on the Fc region, potentially providing higher signal amplification in secondary antibody applications compared to a monoclonal secondary. It is ideal for detecting rat primary antibodies in various immunoassays.
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Why would I choose an Fc-specific secondary antibody over a whole IgG secondary?
A: Choosing an Fc-specific secondary antibody reduces the likelihood of cross-reactivity with the Fab region or light chains of other immunoglobulins, which can be important if you are using multiple antibodies or if there are endogenous immunoglobulins in your sample. It ensures that you are specifically detecting the heavy chain class of your primary antibody, leading to cleaner background in assays like IHC or ELISA.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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