Mouse Anti-RBFOX3 Recombinant Antibody (clone 5A4B9) (CAT#: VS7-0425-WR771)
This product is a mouse IgG1 antibody that binds to human RBFOX3. The antibody 5A4B9 recognizes specific amino acid domain of human RBFOX3 (AA: 1-140). It can be used for WB, ICC, and FC assays. It cross reacts with mouse.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30790/if_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30790/if_selected.jpg
Figure 2 High expression in cerebral cortex
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30790/67653_B_7_5.jpg
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30790/67653_B_7_5.jpg
Figure 3 Colon
(Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30790/67653_A_9_3.jpg
(Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30790/67653_A_9_3.jpg
Figure 4 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30790/67653_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30790/67653_A_8_5.jpg
Figure 5 RNA cell line category: Group enriched (A549, NTERA-2, SCLC-21H, U-2 OS)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167281-RBFOX3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167281-RBFOX3
Specifications
- Immunogen
- Recombinant human RBFOX3 (AA: 1-140) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human RBFOX3
- Species Reactivity
- Human, Mouse
- Clone
- 5A4B9
- Molecule Class
- RNA-Binding Protein
- Applications
- WB, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- RNA Binding Fox-1 Homolog 3; Hexaribonucleotide Binding Protein 3; RNA Binding Protein, Fox-1 Homolog 3; Neuronal Nuclei Antigen; Fox-1 Homolog C; NeuN Antigen; RNA Binding Protein, Fox-1 Homolog (C. Elegans) 3
- Gene ID
- 146713
- UniProt ID
- A6NFN3
- Long Name
- RNA Binding Fox-1 Homolog 3
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Methylation
- Protein Refseq
- NP_001076044.1
- Function
- RNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2427CT | Recombinant Mouse anti-Human RBFOX3 Monoclonal antibody (1B7) | WB, ICC/IF | |
| MRO-1076-CN | Recombinant Rabbit Anti-RBFOX3 Monoclonal Antibody (CBACN-398) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ110 | Rabbit Anti-RBFOX3 Recombinant Antibody (VS3-CJ110) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS-0223-WK50 | Rabbit Anti-RBFOX3 Recombinant Antibody (VS-0223-WK50) | ICC, IHC, IHC-P | Rabbit IgG |
| VS7-0425-WR772 | Mouse Anti-RBFOX3 Recombinant Antibody (clone 1H3B2) | WB, ICC, FC | Mouse IgG2a |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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