CytoStream™ Rabbit Anti-RBFOX3 Recombinant Antibody (VS13-YC989)
CAT#: VS13-YC989
The CytoStream™ Rabbit Anti-RBFOX3 Recombinant Antibody is specific for human, mouse and rat RBFOX3. RBFOX3 encodes an RNA-binding protein regulating splicing and differentiation in neural tissues, linked to disorders via mutations. The anti-RBFOX3 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30790/if_selected.jpg
Normal Tissue
Figure 2 High expression in cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30790/67653_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30790/67653_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30790/67653_A_8_5.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (A549, NTERA-2, SCLC-21H, U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167281-RBFOX3
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Specifications
- Immunogen
- Recombinant human NeuN, aa 20-60.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat RBFOX3
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500
Immunocytochemistry (ICC): 1/50
Immunofluorescence (IF): 1/50
Immunohistochemistry-paraffin (IHC-P): 1/200-1/500
Flow cytometry (FC): 1/50
Target
- Alternative Names
- RNA Binding Fox-1 Homolog 3; Hexaribonucleotide Binding Protein 3; RNA Binding Protein, Fox-1 Homolog 3; Neuronal Nuclei Antigen; Fox-1 Homolog C; NeuN Antigen; RNA Binding Protein, Fox-1 Homolog (C. Elegans) 3;
- Gene ID
- 146713
- UniProt ID
- A6NFN3
- Long Name
- RNA Binding Fox-1 Homolog 3
- Cellular Localization
- Nucleus; Cytoplasm
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "RBFOX3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0729MZ | Chicken Anti-RBFOX3 Polyclonal IgY | ICC, IF, IHC | Chicken antibody |
| BRD-0745MZ | Chicken Anti-NeuN Chicken IgY Polyclonal IgY | IHC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3020 | Hi-Affi™ Recombinant Rabbit Anti-RBFOX3 Monoclonal Antibody (DS3020AB) | IHC-Fr, IHC-P, WB, ICC, IF | IgG |
| MOR-0102-FY | Rabbit Anti-RBFOX3 Recombinant Antibody (clone AFY0073) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1076-CN | Recombinant Rabbit Anti-RBFOX3 Monoclonal Antibody (CBACN-398) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ110 | Rabbit Anti-RBFOX3 Recombinant Antibody (VS3-CJ110) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS3-FY1256 | Recombinant Rabbit Anti-RBFOX3 Antibody (clone R09-7H2) | WB, ICC, IF | Rabbit IgG |
| VS7-0425-WR771 | Mouse Anti-RBFOX3 Recombinant Antibody (clone 5A4B9) | WB, ICC, FC | Mouse IgG1 |
| VS7-0425-WR772 | Mouse Anti-RBFOX3 Recombinant Antibody (clone 1H3B2) | WB, ICC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6027 | Anti-RBFOX3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6028 | Anti-Mouse RBFOX3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6029 | Anti-Rat RBFOX3 Immunohistochemistry Kit | IHC |
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