Rabbit Anti-RCC1 Recombinant Antibody (clone R04-4A8)
CAT#: VS3-FY2078
This product is a rabbit recombinant monoclonal antibody that is designed to compete for binding to human RCC1. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. RCC1 antibody can be applied to in immunocytochemistry analysis of RCC1 in Hela.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27573/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27574/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15413/if_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27574/ihc_selected.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27573/60178_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27573/60178_A_8_4.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27573/60178_A_9_8.jpg
Normal Tissue
Figure 8 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27573/60178_B_8_5.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180198-RCC1
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ICC
Figure 1 Anti-RCC1 Antibody (VS3-FY2078) in ICC.
Immunocytochemistry staining of RCC1 (green) in Hela using the anti-RCC1 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Specifications
- Immunogen
- Synthetic peptide of human RCC1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human RCC1
- Species Reactivity
- Human
- Clone
- R04-4A8
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 45 kDa, while the observed molecular weight stands at 45 kDa.
- Related Disease
- Renal-Hepatic-Pancreatic Dysplasia and Retinitis Pigmentosa
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Regulator Of Chromosome Condensation 1; Cell Cycle Regulatory Protein; CHC1; Guanine Nucleotide-Releasing Protein; Regulator Of Chromosome Condensation; Chromosome Condensation Protein 1
- Gene ID
- 1104
- UniProt ID
- P18754
- Cellular Localization
- Chromosome, Cytoplasm, Nucleus
- Post Translation Modifications
- N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association.
Di- and trimethylation produce a permanent positive charge on the amino group, which facilitates electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding.
Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
(P18754-RCC1_HUMAN)
Ubiquitination at Lys161 and Lys335 (NX_P18754 [NX_P18754-1])
Modification sites at PhosphoSitePlus (P18754)
Glycosylation from GlyGen (P18754) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_001041659.1; NP_001041660.1; NP_001041664.1; NP_001260.1; NP_001368794.1
- Function
- Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis.
Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RCC1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1889z | Mouse Anti-RCC1 Recombinant Antibody (clone 31C8) | WB, ELISA, ICC, IF, IHC | Mouse IgG1, κ |
| MOB-1781CT | Recombinant Mouse anti-Human RCC1 Monoclonal antibody (EML042) | IHC-P, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3034 | Hi-Affi™ Recombinant Rabbit Anti-RCC1 Monoclonal Antibody (DS3034AB) | ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC995 | CytoStream™ Rabbit Anti-RCC1 Recombinant Antibody (VS13-YC995) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1899 | Anti-RCC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6054 | Anti-Mouse RCC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6053 | Anti-Human RCC1 Immunohistochemistry Kit | IHC |
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