Rabbit Anti-RCC1 Recombinant Antibody (clone R04-4A8) (CAT#: VS3-FY2078)

• Immunogen
• Synthetic peptide of human RCC1

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human RCC1

• Species Reactivity
• Human

• Clone
• R04-4A8

• Applications
• Western Blot, Immunofluorescence, Immunoprecipitation

• MW
• The calculated weight of the target protein is approximately 45 kDa, while the observed molecular weight stands at 45 kDa.

• Related Disease
• Renal-Hepatic-Pancreatic Dysplasia and Retinitis Pigmentosa

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunofluorescence: 1/50-1/200
  Immunoprecipitation: 1/20

• Alternative Names
• Regulator Of Chromosome Condensation 1; Cell Cycle Regulatory Protein; CHC1; Guanine Nucleotide-Releasing Protein; Regulator Of Chromosome Condensation; Chromosome Condensation Protein 1

• Gene ID
• 1104

• UniProt ID
• P18754

• Cellular Localization
• Chromosome, Cytoplasm, Nucleus

• Post Translation Modifications
• N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association.
  Di- and trimethylation produce a permanent positive charge on the amino group, which facilitates electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding.
  Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
  (P18754-RCC1_HUMAN)
  Ubiquitination at Lys161 and Lys335 (NX_P18754 [NX_P18754-1])
  Modification sites at PhosphoSitePlus (P18754)
  Glycosylation from GlyGen (P18754) 2 sites, 1 O-linked glycan (2 sites)

• Protein Refseq
• NP_001041659.1; NP_001041660.1; NP_001041664.1; NP_001260.1; NP_001368794.1

• Function
• Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis.
  Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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