Mouse Anti-RCHY1 Monoclonal Antibody (VS7-0425-WR774) (CAT#: VS7-0425-WR774)
This product is a mouse IgG antibody that binds to human RCHY1. The antibody can be used for WB, IHC-P, IF, ICC, FC, and ELISA assays. It cross reacts with rat.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30338/1893_H21_27_cr5bbdf6f89e5b1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30338/1893_H21_27_cr5bbdf6f89e5b1_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30339/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30339/ihc_selected.jpg
Figure 3 Stomach
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30339/68918_A_3_1.jpg
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30339/68918_A_3_1.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30339/68918_A_9_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30339/68918_A_9_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30339/68918_A_8_5.jpg
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30339/68918_A_8_5.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163743-RCHY1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163743-RCHY1
Specifications
- Immunogen
- Recombinant human RCHY1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human RCHY1
- Species Reactivity
- Human, Rat
- Molecule Class
- E3 Ubiquitin Ligase
- Applications
- WB, IHC-P, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Immunofluorescence: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Target
- Alternative Names
- Ring Finger And CHY Zinc Finger Domain Containing 1; ZNF363; Androgen-Receptor N-Terminal-Interacting Protein; P53-Induced Protein With A RING-H2 Domain; RING-Type E3 Ubiquitin Transferase RCHY1; E3 Ubiquitin-Protein Ligase Pirh2; Zinc Finger Protein 363; RING Finger Protein 199; Zinc Finger, CHY-Type; RNF199; ARNIP; CHIMP
- Gene ID
- 25898
- UniProt ID
- Q96PM5
- Long Name
- Ring Finger And CHY Zinc Finger Domain Containing 1
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001009922.1
- Function
- Transferase
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1606CT | Recombinant Mouse anti-Human RCHY1 Monoclonal antibody (EML1415) | WB | |
| VS3-QX965 | Mouse Anti-RCHY1 Recombinant Antibody (clone 7B9-H8-D2) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY1262 | Recombinant Rabbit Anti-RCHY1 Antibody (clone R06-8H6) | WB | Rabbit IgG |
| VS7-0425-WR618 | Mouse Anti-RCHY1 Monoclonal Antibody (clone 1H10) | WB, IHC, ICC, FC | Mouse IgG1 |
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