Mouse Anti-RCHY1 Monoclonal Antibody (VS7-0425-WR774)
CAT#: VS7-0425-WR774
This product is a mouse IgG antibody that binds to human RCHY1. The antibody can be used for WB, IHC-P, IF, ICC, FC, and ELISA assays. It cross reacts with rat.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30338/1893_H21_27_cr5bbdf6f89e5b1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30339/ihc_selected.jpg
Normal Tissue
Figure 3 Stomach
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30339/68918_A_3_1.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30339/68918_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30339/68918_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163743-RCHY1
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Specifications
- Immunogen
- Recombinant human RCHY1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human RCHY1
- Species Reactivity
- Human, Rat
- Molecule Class
- E3 Ubiquitin Ligase
- Applications
- WB, IHC-P, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Immunofluorescence: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Target
- Alternative Names
- Ring Finger And CHY Zinc Finger Domain Containing 1; ZNF363; Androgen-Receptor N-Terminal-Interacting Protein; P53-Induced Protein With A RING-H2 Domain; RING-Type E3 Ubiquitin Transferase RCHY1; E3 Ubiquitin-Protein Ligase Pirh2; Zinc Finger Protein 363; RING Finger Protein 199; Zinc Finger, CHY-Type; RNF199; ARNIP; CHIMP
- Gene ID
- 25898
- UniProt ID
- Q96PM5
- Long Name
- Ring Finger And CHY Zinc Finger Domain Containing 1
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001009922.1
- Function
- Transferase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "RCHY1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1606CT | Recombinant Mouse anti-Human RCHY1 Monoclonal antibody (EML1415) | WB | |
| VS3-QX965 | Mouse Anti-RCHY1 Recombinant Antibody (clone 7B9-H8-D2) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY1262 | Recombinant Rabbit Anti-RCHY1 Antibody (clone R06-8H6) | WB | Rabbit IgG |
| VS7-0425-WR618 | Mouse Anti-RCHY1 Monoclonal Antibody (clone 1H10) | WB, IHC, ICC, FC | Mouse IgG1 |
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