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  • Rabbit Anti-RNF7 Recombinant Antibody (clone R24-5V-6)

Rabbit Anti-RNF7 Recombinant Antibody (clone R24-5V-6) (CAT#: VS3-FY2186)

This R24-5V-6 recombinant monoclonal antibody shows a high affinity for human RNF7 protein, a calculated weight of 13 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R24-5V-6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200).


See more Engineered Antibody products for RNF7
Specific Inquiry
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  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36995/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Figure 2 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36995/ihc_selected.jpg
Figure 3 Colon
Figure 3 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36995/74350_A_9_3.jpg
Figure 4 Liver
Figure 4 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36995/74350_A_7_4.jpg
Figure 5 Kidney
Figure 5 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36995/74350_A_8_5.jpg
Figure 6 Testis
Figure 6 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36995/74350_A_5_6.jpg
Figure 7 Bone marrow
Figure 7 Bone marrow
(Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36995/74350_B_5_4.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000114125-RNF7

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat RNF7
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R24-5V-6
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
  • Conjugate
  • Unconjugated
  • MW
  • The calculated weight of the target protein is approximately 13kDa, while the observed molecular weight stands at 13kDa.
  • Related Disease
  • Brachydactyly, Type E2 and Lung Cancer

Product Property

  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl, 50% glycerol
  • Preservative
  • 0.02% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/100-1/200
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • Ring Finger Protein 7; Sensitive To Apoptosis, Zinc RING Finger Protein SAG, Regulator Of Cullins 2; Sensitive To Apoptosis Gene Protein; CKII Beta-Binding Protein 1; Regulator Of Cullins 2; RING Finger Protein 7; CKBBP1
  • Sequence Similarities
  • Belongs to the RING-box family.
  • Cellular Localization
  • Cytoplasm, Nucleus
  • Post Translation Modifications
  • Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B. (Q9UBF6-RBX2_HUMAN)
    Modification sites at PhosphoSitePlus (Q9UBF6)
    Modification sites at neXtProt (NX_Q9UBF6)
    Glycosylation from GlyGen (Q9UBF6) 1 site, 1 O-linked glycan (1 site)
  • Function
  • Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription.
    CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (By similarity).

Recommended Products

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3089 Hi-Affi™ Recombinant Rabbit Anti-RNF7 Monoclonal Antibody (DS3089AB) IHC-P, WB, ICC/IF IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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