Mouse Anti-RPA2 Recombinant Antibody (clone 2C5B6) (CAT#: VS7-0425-WR779)
This product is a mouse IgG1,kappa antibody that binds to human RPA2. The antibody 2C5B6 can be used for WB, IHC, and FC assays.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26306/220_B6_1_selected.jpg

(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_8_5.jpg

(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_6_6.jpg

(Cells in basal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity:>75% Endothelial cells Staining: Medium Intensity: Moderate Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: Medium Intensity: Moderate Quantity:>75% Lymphocytes Staining: High Intensity: Strong Melanocytes Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_B_7_1.jpg

(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_9_8.jpg

(Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_9_3.jpg

(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_8_4.jpg

(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117748-RPA2
Specifications
- Immunogen
- Recombinant human RPA2 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1,kappa
- Specificity
- Human RPA2
- Species Reactivity
- Human
- Clone
- 2C5B6
- Molecule Class
- Replication Protein A (RPA) Complex
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Target
- Alternative Names
- Replication Protein A2; Replication Protein A 34 KDa Subunit; Replication Factor A Protein 2; RF-A Protein 2; RP-A P32; RP-A P34; REPA2
- Gene ID
- 6118
- UniProt ID
- P15927
- Long Name
- Replication Protein A2
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001273005.1
- Function
- DNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "RPA2"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2562z | Mouse Anti-RPA2 Recombinant Antibody (clone 30F9) | WB, ICC, IF, IHC, IP | Mouse IgG1, κ |
VS3-WK367 | Rabbit Anti-RPA2 Recombinant Antibody (clone JE45-59) | WB, IP, IHC | Rabbit IgG |
VS3-CJ680 | Mouse Anti-RPA2 Recombinant Antibody (VS3-CJ680) | WB, IHC | Mouse IgG |
VS3-CJ681 | Rabbit Anti-RPA2 (phospho T21) Recombinant Antibody (VS3-CJ681) | WB, IP | Rabbit IgG |
VS3-WK2133 | Rabbit Anti-RPA2 (pT21) Recombinant Antibody (clone R02-2I5) | WB, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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