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    Mouse Anti-RPA2 Recombinant Antibody (clone 2C5B6) (CAT#: VS7-0425-WR779)

    This product is a mouse IgG1,kappa antibody that binds to human RPA2. The antibody 2C5B6 can be used for WB, IHC, and FC assays.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
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    Fc Engineering :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26306/220_B6_1_selected.jpg
    Normal Tissue
    Figure 2 Kidney
    Figure 2 Kidney
    (Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_8_5.jpg
    Figure 3 Testis
    Figure 3 Testis
    (Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_6_6.jpg
    Figure 4 Skin
    Figure 4 Skin
    (Cells in basal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity:>75% Endothelial cells Staining: Medium Intensity: Moderate Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: Medium Intensity: Moderate Quantity:>75% Lymphocytes Staining: High Intensity: Strong Melanocytes Staining: High Intensity: Strong Quantity: 75%-25%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_B_7_1.jpg
    Figure 5 Lymph node
    Figure 5 Lymph node
    (Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_9_8.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_9_3.jpg
    Figure 7 Liver
    Figure 7 Liver
    (Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_8_4.jpg
    RNA Expression
    Figure 8 RNA cell line category: Low cell line specificity
    Figure 8 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117748-RPA2

    Specifications

    • Immunogen
    • Recombinant human RPA2 fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1,kappa
    • Specificity
    • Human RPA2
    • Species Reactivity
    • Human
    • Clone
    • 2C5B6
    • Molecule Class
    • Replication Protein A (RPA) Complex
    • Applications
    • WB, IHC, FC
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Immunohistochemistry-Paraffin: 1:200 - 1:1000
      Flow Cytometry: 1:200 - 1:400

    Target

    • Alternative Names
    • Replication Protein A2; Replication Protein A 34 KDa Subunit; Replication Factor A Protein 2; RF-A Protein 2; RP-A P32; RP-A P34; REPA2
    • Long Name
    • Replication Protein A2
    • Cellular Localization
    • Nucleus
    • Post Translation Modifications
    • Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
    • Function
    • DNA-binding

    Recommended Products

    Isotype Control

    Secondary Antibody

    Reconstitution Buffer

    Recommended Dilution Buffer

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "RPA2"

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    MOB-2562z Mouse Anti-RPA2 Recombinant Antibody (clone 30F9) WB, ICC, IF, IHC, IP Mouse IgG1, κ
    VS3-WK367 Rabbit Anti-RPA2 Recombinant Antibody (clone JE45-59) WB, IP, IHC Rabbit IgG
    VS3-CJ680 Mouse Anti-RPA2 Recombinant Antibody (VS3-CJ680) WB, IHC Mouse IgG
    VS3-CJ681 Rabbit Anti-RPA2 (phospho T21) Recombinant Antibody (VS3-CJ681) WB, IP Rabbit IgG
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