Mouse Anti-RPA2 Recombinant Antibody (clone 2C5B6)
CAT#: VS7-0425-WR779
This product is a mouse IgG1,kappa antibody that binds to human RPA2. The antibody 2C5B6 can be used for WB, IHC, and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26306/220_B6_1_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_8_5.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_6_6.jpg
Normal Tissue
Figure 4 Skin
Cells in basal layer Staining: Medium Intensity: Moderate Quantity:>75% Cells in granular layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity:>75% Endothelial cells Staining: Medium Intensity: Moderate Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: Medium Intensity: Moderate Quantity:>75% Lymphocytes Staining: High Intensity: Strong Melanocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_B_7_1.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_9_8.jpg
Normal Tissue
Figure 6 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16538/36325_A_8_4.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000117748-RPA2
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Specifications
- Immunogen
- Recombinant human RPA2 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1,kappa
- Specificity
- Human RPA2
- Species Reactivity
- Human
- Clone
- 2C5B6
- Molecule Class
- Replication Protein A (RPA) Complex
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Target
- Alternative Names
- Replication Protein A2; Replication Protein A 34 KDa Subunit; Replication Factor A Protein 2; RF-A Protein 2; RP-A P32; RP-A P34; REPA2
- Gene ID
- 6118
- UniProt ID
- P15927
- Long Name
- Replication Protein A2
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001273005.1
- Function
- DNA-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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