Rabbit Anti-RPS20 Recombinant Antibody (VS3-FY2864)
CAT#: VS3-FY2864
The anti-RPS20 antibody is a rabbit recombinant monoclonal antibody that with high affinity for human RPS20 protein. It cross-reacts with mouse, rat, hamster species. This monoclonal antibody proves valuable in disease research related to Diamond-Blackfan Anemia and Familial Colorectal Cancer Type X. The antibody can be utilized for detection of target protein by Western Blot (1/500-1/1000), Immunohistochemistry (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3570/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human oral mucosa
Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3570/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3570/10533_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3570/10533_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3570/10533_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3570/10533_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000008988-RPS20
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat and Hamster RPS20
- Species Reactivity
- Human, Mouse, Rat, Hamster
- Applications
- Western Blot, Immunohistochemistry
- Conjugate
- Unconjugated, FITC
- Related Disease
- Diamond-Blackfan Anemia and Familial Colorectal Cancer Type X
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Ribosomal Protein S20; Small Ribosomal Subunit Protein US10; 40S Ribosomal Protein S20; S20
- Gene ID
- 6224
- UniProt ID
- P60866
- Sequence Similarities
- Belongs to the universal ribosomal protein uS10 family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Polyubiquitinated by ZNF598 via 'Lys-63'-linked ubiquitin chains when a ribosome has stalled, initiating the ribosome quality control (RQC) pathway to degrade the potentially detrimental aberrant nascent polypeptide (PubMed:28065601, 28132843, 28685749, 32011234, 36302773).
Deubiquitinated by OTUD3 and USP21, antagonizing ZNF598 activity (PubMed:32011234).
(P60866-RS20_HUMAN)
Ufmylated by UFL1. (P60866-RS20_HUMAN)
Ubiquitination at Lys4, Lys30, Lys34, Lys67, and Lys75 (NX_P60866 [NX_P60866-1])
Modification sites at PhosphoSitePlus (P60866)
Glycosylation from GlyGen (P60866) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001139699.1
- Function
- Component of the small ribosomal subunit (PubMed:23636399).
The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "RPS20"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3118 | Hi-Affi™ Recombinant Rabbit Anti-RPS20 Monoclonal Antibody (DS3118AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1347-CN | Recombinant Rabbit Anti-RPS20 Monoclonal Antibody (CBACN-489) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ1068 | Rabbit Anti-RPS20 Recombinant Antibody (VS3-CJ1068) | WB, ICC, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1016 | Rabbit Anti-RPS20 Recombinant Antibody (VS13-YC1016) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1962 | Anti-RPS20 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6211 | Anti-Mouse RPS20 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6210 | Anti-Human RPS20 Immunohistochemistry Kit | IHC |
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