Rabbit Anti-RPS6KA2 Recombinant Antibody (clone R05-5E4)
CAT#: VS3-FY1946
Clone R05-5E4 is a rabbit recombinant monoclonal antibody that shows a high affinity for human RPS6KA2. It cross-reacts with hamster. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We validate the QC result of the antibody using western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20). The antibody has the positive detection of RSK3 protein by western blot analysis of RSK3 in CHO-K1, Hela lysates and immunohistochemistry analysis of paraffin-embedded human lung cancer tissue.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45061/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45061/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human vagina
Immunohistochemical staining of human vagina shows moderate cytoplasmic and nuclear membranous positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54237/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human oral mucosa
Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26243/55000_A_7_1_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69428/149225_A_7_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69428/149225_B_9_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69428/149225_A_7_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69428/149225_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69428/149225_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, HHSteC, HSkMC, hTERT-HME1, hTERT-RPE1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000071242-RPS6KA2
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WB
Figure 1 Anti-RPS6KA2 Antibody (VS3-FY1946) in WB.
The RSK3 expression in CHO-K1, Hela lysates were detected using the anti-RSK3 by western blot assay.
IHC
Figure 2 Anti-RPS6KA2 Antibody (VS3-FY1946) in IHC.
Immunohistochemistry staining of paraffin-embedded human lung cancer tissue using the anti-RSK3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human RSK3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Hamster RPS6KA2
- Species Reactivity
- Human, Hamster
- Clone
- R05-5E4
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 83 kDa, while the observed molecular weight stands at 90 kDa.
- Related Disease
- Human lung cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Ribosomal Protein S6 Kinase A2; MAP Kinase-Activated Protein Kinase 1c; MAPK-Activated Protein Kinase 1c; Ribosomal S6 Kinase 3; EC 2.7.11.1; MAPKAPK1C; Pp90RSK3; P90RSK2; RSK3; Mitogen-Activated Protein Kinase-Activated Protein Kinase 1C; Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 2; Ribosomal Protein S6 Kinase, 90kD, Polypeptide 2; 90 KDa Ribosomal Protein S6 Kinase 2; Ribosomal Protein S6 Kinase Alpha-2
- Gene ID
- 6196
- UniProt ID
- Q15349
- Sequence Similarities
- Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Activated by phosphorylation at Ser-218 by PDPK1.
Autophosphorylated on Ser-377, as part of the activation process.
May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1 ().
(Q15349-KS6A2_HUMAN)
N-terminal myristoylation results in an activated kinase in the absence of added growth factors. (Q15349-KS6A2_HUMAN)
Modification sites at PhosphoSitePlus (Q15349)
Modification sites at neXtProt
- Protein Refseq
- XP_006715612.1; XP_047275189.1; XP_047275190.1; XP_047275191.1; XP_054212163.1
- Function
- Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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