This product is a recombinant humanized antibody that recognizes serum albumin. The binding reactivity to human SA, rat SA and mouse SA was tested in ELISA. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Figure 1 Human Anti-SA Antibody (HPAB-2545LY) in SDS-PAGE
SDS-PAGE analysis of HPAB-2545LY in non-reduced (lane 2) and reduced (lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 2 Human Anti-SA Antibody (HPAB-2545LY) in HPLC
The purity of this antibody is> 95% as determined by SEC-HPLC.
Figure 3 Human Anti-SA Antibody (HPAB-2545LY) in WB
Western blot analysis of HPAB-2545LY was performed by loading Human SA Protein.
TAB-422CQ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)
Lane 1: Reducing Antigen (0.6 μg)
Lane 2: Reducing Antigen (0.3 μg)
Figure 4 Anti-Human SA Antibody (HPAB-2545LY) in ELISA
ELISA analysis of HPAB-2545LY was performed by coating with Human SA Protein. Then blocked with BSA and incubated with HPAB-2545LY. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 5 Anti-Human SA Antibody (HPAB-2545LY) in DB
Dot Blot analysis of HPAB-2545LY was performed by coating with Recombinant Human SA Protein.
HPAB-2545LY incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-2540LY-F(E) | Human Anti-SA Recombinant Antibody; Fab Fragment (HPAB-2540LY-F(E)) | ELISA, WB | Humanized Fab |
HPAB-2541LY-F(E) | Human Anti-SA Recombinant Antibody; Fab Fragment (HPAB-2541LY-F(E)) | ELISA, WB | Humanized Fab |
HPAB-2542LY-F(E) | Human Anti-SA Recombinant Antibody; Fab Fragment (HPAB-2542LY-F(E)) | ELISA, WB | Humanized Fab |
HPAB-2543LY-F(E) | Human Anti-SA Recombinant Antibody; Fab Fragment (HPAB-2543LY-F(E)) | ELISA, WB | Humanized Fab |
HPAB-2544LY-F(E) | Human Anti-SA Recombinant Antibody; Fab Fragment (HPAB-2544LY-F(E)) | ELISA, WB | Humanized Fab |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-2545LY, RRID: AB_3111426)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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