Rabbit Anti-SALL4 Recombinant Antibody (clone R02-6H7)
CAT#: VS3-FY1874
The product is meticulously crafted to specifically target human SALL4 by WB and IHC assay. It is a rabbit recombinant monoclonal antibody that was purified by affinity chromatography. The R02-6H7 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15291/1030_F9_5_red_green.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15291/1183_B10_1_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15291/35651_A_6_6.jpg
Normal Tissue
Figure 4 Ovary
Follicle cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15291/35651_A_5_7.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (AF22, AN3-CA, BEWO, NTERA-2, SiHa, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000101115-SALL4
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Specifications
- Immunogen
- Recombinant human Sall4 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SALL4
- Species Reactivity
- Human
- Clone
- R02-6H7
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 112 kDa, while the observed molecular weight stands at 112 kDa.
- Related Disease
- Duane-Radial Ray Syndrome and Ivic Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Spalt Like Transcription Factor 4; Zinc Finger Protein SALL4; Zinc Finger Protein 797; ZNF797; Sal (Drosophila)-Like 4; Sal-Like 4 (Drosophila); Sal-Like Protein 4; HSAL4; DRRS
- Gene ID
- 57167
- UniProt ID
- Q9UJQ4
- Sequence Similarities
- Belongs to the sal C2H2-type zinc-finger protein family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Isoform SALL4B exists primarily as a ubiquitinated form. (Q9UJQ4-SALL4_HUMAN)
Sumoylation with both SUMO1 and SUMO2 regulates the stability, subcellular localization, transcriptional activity, and may reduce interaction with POU5F1/OCT4. (Q9UJQ4-SALL4_HUMAN)
Modification sites at PhosphoSitePlus (Q9UJQ4)
Modification sites at neXtProt (NX_Q9UJQ4)
Glycosylation from GlyGen (Q9UJQ4) 3 sites, 1 O-linked glycan (3 sites)
- Protein Refseq
- XP_047296274.1; XP_054179717.1; NP_001304960.1; NP_065169.1
- Function
- Transcription factor with a key role in the maintenance and self-renewal of embryonic and hematopoietic stem cells.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SALL4"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-505 | Recombinant Human Anti-Human SALL4 Antibody Fab Fragment, clone D202 | IP, IF, ELISA | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1823CT | Recombinant Mouse anti-Human SALL4 Monoclonal antibody (EML1753) | FC, IHC-P, WB | |
| MOB-0241F | Mouse Anti-SALL4 Recombinant Antibody (MOB-0241F) | IHC-P, IF | Mouse IgG |
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| VS3-FY1306 | Recombinant Mouse Anti-SALL4 Antibody (clone 1A12C3) | ELISA, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC89 | Rabbit Anti-SALL4 Antibody (VS-0924-YC89) - Cancer Stem Cell Marker | IHC, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6341 | Anti-SALL4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6342 | Anti-Mouse SALL4 Immunohistochemistry Kit | IHC |
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