Rabbit Anti-SALL4 Recombinant Antibody (clone R02-6H7) (CAT#: VS3-FY1874)
The product is meticulously crafted to specifically target human SALL4 by WB and IHC assay. It is a rabbit recombinant monoclonal antibody that was purified by affinity chromatography. The R02-6H7 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100).
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15291/1030_F9_5_red_green.jpg
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15291/1030_F9_5_red_green.jpg
Figure 2 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15291/1183_B10_1_red_green.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15291/1183_B10_1_red_green.jpg
Figure 3 Cerebral cortex
(Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15291/35651_A_6_6.jpg
(Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15291/35651_A_6_6.jpg
Figure 4 Ovary
(Follicle cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15291/35651_A_5_7.jpg
(Follicle cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15291/35651_A_5_7.jpg
Figure 5 RNA cell line category: Cell line enhanced (AF22, AN3-CA, BEWO, NTERA-2, SiHa, SuSa)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000101115-SALL4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000101115-SALL4
Specifications
- Immunogen
- Recombinant human Sall4 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SALL4
- Species Reactivity
- Human
- Clone
- R02-6H7
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 112 kDa, while the observed molecular weight stands at 112 kDa.
- Related Disease
- Duane-Radial Ray Syndrome and Ivic Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Spalt Like Transcription Factor 4; Zinc Finger Protein SALL4; Zinc Finger Protein 797; ZNF797; Sal (Drosophila)-Like 4; Sal-Like 4 (Drosophila); Sal-Like Protein 4; HSAL4; DRRS
- Gene ID
- 57167
- UniProt ID
- Q9UJQ4
- Sequence Similarities
- Belongs to the sal C2H2-type zinc-finger protein family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Isoform SALL4B exists primarily as a ubiquitinated form. (Q9UJQ4-SALL4_HUMAN)
Sumoylation with both SUMO1 and SUMO2 regulates the stability, subcellular localization, transcriptional activity, and may reduce interaction with POU5F1/OCT4. (Q9UJQ4-SALL4_HUMAN)
Modification sites at PhosphoSitePlus (Q9UJQ4)
Modification sites at neXtProt (NX_Q9UJQ4)
Glycosylation from GlyGen (Q9UJQ4) 3 sites, 1 O-linked glycan (3 sites)
- Protein Refseq
- XP_047296274.1; XP_054179717.1; NP_001304960.1; NP_065169.1
- Function
- Transcription factor with a key role in the maintenance and self-renewal of embryonic and hematopoietic stem cells.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-505 | Recombinant Human Anti-Human SALL4 Antibody Fab Fragment, clone D202 | IP, IF, ELISA | Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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