Mouse Anti-SARS-CoV-2 (2019-nCoV) S RBD Recombinant Antibody (clone CBS351)
CAT#: MRO-A1819-YJ
This product is a Monoclonal mouse antibody that recognizes SARS-CoV-2 (2019-nCoV) S RBD, N501Y (B.1.1.7, Alpha Variant), RBD L452R, E484Q (B.1.617, B.1.617.1, B.1.617.3), RBD L452R, T478K (B.1.617.2, Delta Variant). It has no cross-reactivity in ELISA with SARS-CoV Spike S1+S2 ECD Protein, MERS-CoV Spike S1+S2 ECD Protein, HCoV-NL63 Spike S1+S2 ECD Protein, HCoV-229E Spike S1+S2 ECD Protein, HCoV-HKU1 (isolate N5) Spike S1+S2 ECD Protein, HCoV-OC43 Spike S1+S2 ECD Protein, RBD K417N, E484K, N501Y (B.1.351, Beta Variant), RBD K417T, E484K, N501Y (P.1, Gamma Variant).
Specifications
- Immunogen
- Recombinant SARS-CoV-2 Spike RBD-his Protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- SARS-CoV-2 (2019-nCoV) S RBD
- Species Reactivity
- SARS-CoV-2 (2019-nCoV)
- Clone
- CBS351
- Applications
- ELISA, IHC-P, FC, ICC, IF, Neut
- Conjugate
- Unconjugated
- Related Disease
- Coronavirus disease, Coronavirus disease 2019
Product Property
- Purification
- Protein A affinity chromatography
- Format
- Liquid
- Buffer
- PBS
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Neutralization.
Target
- Alternative Names
- SARS-CoV Spike Protein; SARS-CoV; SARS-CoV S protein; SARS-CoV Spike; 2019-nCoV Spike Protein RBD Domain; 2019-nCoV; COVID-19; S1 domain of the 2019-nCoV S protein; 2019-nCoV Spike Protein S1 Domain; ncov s1; NCP-CoV Spike; NCP-CoV s1; SARS-CoV Spike Protein S1 Domain; novel coronavirus RBD; SARS-CoV-2; spike; SARS; SARS-CoV; RBD; receptor-binding domain
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Reliable for Variant Detection
This Mouse Anti-SARS-CoV-2 S RBD Antibody has been a pivotal addition to our lab, particularly for detecting different variants of the virus. Its high specificity for the spike protein, especially against the Alpha and Delta variants, allows us to carry out precise studies on viral mutations. This antibody has consistently delivered reliable results, reinforcing the quality of our virological research.
High Affinity and Specificity
We have extensively used this antibody for ELISA and immunofluorescence, and its performance has been outstanding. The high affinity for the spike protein coupled with its purity results in low background and clear signals, facilitating high-confidence data in our assays, essential for progressing our viral research and understanding immune interactions.
Great Tool for Neutralization Assays
The Mouse Anti-SARS-CoV-2 S RBD antibody has become indispensable for our neutralization assays. Its robust specificity and ability to neutralize the spike protein allow for accurate assessments of antiviral agents and vaccine candidates, making it a critical resource for advancing our work on viral therapeutics.
Q&As
-
What is the main application of the Mouse Anti-SARS-CoV-2 S RBD Recombinant Antibody?
A: This antibody is primarily used for applications like ELISA, immunohistochemistry (IHC), flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), and neutralization (Neut). It's widely utilized in research on SARS-CoV-2, particularly focusing on the spike protein.
-
What makes this antibody immune from cross-reactivity?
A: The antibody shows no cross-reactivity in ELISA with other coronavirus spike proteins such as SARS-CoV, MERS-CoV, and common human coronaviruses like HCoV-NL63 and HCoV-OC43. This ensures precise targeting of the SARS-CoV-2 spike receptor-binding domain (RBD).
-
Can this antibody be used for functional inhibition studies?
A: Yes, aside from detection in diagnostic assays, its neutralizing capacity makes it suitable for functional inhibition studies, aiding in the research of viral entry mechanisms and potential therapeutic interventions.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| HPAB-0931-YJ-F(E) | Human Anti-SARS-CoV-2 (2019-nCoV) S RBD Recombinant Antibody Fab Fragment (HPAB-0931-YJ-F(E)) | ELISA, Inhib | Human Fab |
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| HPAB-0933-YJ-F(E) | Human Anti-SARS-CoV-2 (2019-nCoV) S RBD Recombinant Antibody Fab Fragment (HPAB-0933-YJ-F(E)) | ELISA, Inhib | Human Fab |
| HPAB-0934-YJ-F(E) | Human Anti-SARS-CoV-2 (2019-nCoV) S RBD Recombinant Antibody Fab Fragment (HPAB-0934-YJ-F(E)) | ELISA, Inhib | Human Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0931-YJ | Human Anti-SARS-CoV-2 (2019-nCoV) S RBD Recombinant Antibody (HPAB-0931-YJ) | ELISA, Inhib | Human IgG1 |
| HPAB-0932-YJ | Human Anti-SARS-CoV-2 (2019-nCoV) S RBD Recombinant Antibody (HPAB-0932-YJ) | ELISA, Inhib | Human IgG1 |
| HPAB-0933-YJ | Human Anti-SARS-CoV-2 (2019-nCoV) S RBD Recombinant Antibody (HPAB-0933-YJ) | ELISA, Inhib | Human IgG1 |
| HPAB-0934-YJ | Human Anti-SARS-CoV-2 (2019-nCoV) S RBD Recombinant Antibody (HPAB-0934-YJ) | ELISA, Inhib | Human IgG1 |
| HPAB-0935-YJ | Human Anti-SARS-CoV-2 (2019-nCoV) S RBD Recombinant Antibody (HPAB-0935-YJ) | ELISA, Inhib | Human IgG1 |
| CAT | Product Name | Application | Type |
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| MRO-A1787-YJ | Camelid Anti-SARS-CoV-2 (2019-nCoV) S RBD Recombinant Antibody (MRO-A1787-YJ) | ELISA | Camelid VHH |
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| MRO-A1790-YJ | Camelid Anti-SARS-CoV-2 (2019-nCoV) S RBD Recombinant Antibody (MRO-A1790-YJ) | ELISA | Camelid VHH |
| MRO-A1791-YJ | Camelid Anti-SARS-CoV-2 (2019-nCoV) S RBD Recombinant Antibody (MRO-A1791-YJ) | ELISA | Camelid VHH |
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