Rabbit Anti-SBDS Recombinant Antibody (clone R02-2G2)
CAT#: VS3-FY2084
The anti-SBDS antibody detected positive in western blot analysis of SBDS in A549, HL-60, U2OS, U87-MG lysates. The recombinant monoclonal antibody is meticulously crafted to specifically target human SBDS. It is suitable for use in a variety of assays, including WB, ICC/IF, IP.
Tested Data
Gene Expression
WB
Figure 1 Anti-SBDS Antibody (VS3-FY2084) in WB.
The SBDS expression in A549, HL-60, U2OS, U87-MG lysates were detected using the anti-SBDS by western blot assay.
ICC
Figure 2 Anti-SBDS Antibody (VS3-FY2084) in ICC.
Immunocytochemistry staining of SBDS (green) in Hela using the anti-SBDS antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28891/273_G10_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28891/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28891/61573_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28891/61573_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28891/61573_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28891/61573_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28891/61573_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126524-SBDS
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Specifications
- Immunogen
- Synthetic peptide of human SBDS
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SBDS
- Species Reactivity
- Human
- Clone
- R02-2G2
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 29 kDa, while the observed molecular weight stands at 29 kDa.
- Related Disease
- Shwachman-Diamond Syndrome 1 and Aplastic Anemia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- SBDS, Ribosome Maturation Factor; SBDS, Ribosome Assembly Guanine Nucleotide Exchange Factor; Shwachman-Bodian-Diamond Syndrome Protein; Shwachman-Bodian-Diamond Syndrome; Ribosome Maturation Protein SBDS; CGI-97; SWDS; SDS
- Gene ID
- 51119
- UniProt ID
- Q9Y3A5
- Sequence Similarities
- Belongs to the SDO1/SBDS family.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys62, Lys90, Lys98, and Lys159 (NX_Q9Y3A5 [NX_Q9Y3A5-1])
Modification sites at PhosphoSitePlus (Q9Y3A5)
Glycosylation from GlyGen (Q9Y3A5) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_057122.2
- Function
- Required for the assembly of mature ribosomes and ribosome biogenesis. Together with EFL1, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Required for normal levels of protein synthesis. May play a role in cellular stress resistance. May play a role in cellular response to DNA damage. May play a role in cell proliferation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SBDS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3176 | Hi-Affi™ Recombinant Rabbit Anti-SBDS Monoclonal Antibody (DS3176AB) | WB, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC833 | AbPlus™ Anti-Sbds Magnetic Beads (VS-0724-YC833) | IP, Protein Purification | |
| VS-0724-YC835 | AbPlus™ Anti-SBDS Magnetic Beads (VS-0724-YC835) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1039 | Rabbit Anti-SBDS Recombinant Antibody (VS13-YC1039) | WB, ICC, IF, IP | Rabbit IgG |
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