Rabbit Anti-SERPINH1 Recombinant Antibody (clone R09-6D6)
CAT#: VS3-XY3501
It is a recombinant rabbit antibody specific for SERPINH1 (Serpin family H member 1), which is involves as a chaperone in the biosynthetic pathway of collagen. This antibody specifically targets human, mouse and rat SERPINH1. This product has been tested using Western Blot and Immunohistochemistry-Paraffin. The recommended dilutions of Western Blot and Immunohistochemistry-Paraffin is 1/500-1/1000 and 1/50-1/100.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29198/1866_A1_36_selected.jpg
Normal Tissue
Figure 2 IHC staining of human vulva/anal skin
Immunohistochemical staining of human vulva/anal skin shows moderate cytoplasmic positivity in epidermal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29198/63002_B_6_8_selected.jpg
Normal Tissue
Figure 3 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4441/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4441/11576_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4441/11576_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4441/11576_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4441/11576_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (CACO-2, fHDF/TERT166, HHSteC)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149257-SERPINH1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat SERPINH1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-6D6
- Applications
- WB, IHC-P
- Related Disease
- Osteogenesis Imperfecta, Type X; Preterm Premature Rupture of The Membranes
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
Target
- Alternative Names
- CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
- Gene ID
- 871
- UniProt ID
- P50454
- Long Name
- Serpin family H member 1
- Cellular Localization
- Endoplasmic reticulum lumen
- Post Translation Modifications
- Glycosylation at Asn120 and Asn125 (NX_P50454 [NX_P50454-1])
Ubiquitination at Lys39, Lys94, Lys319, and Lys380 (NX_P50454 [NX_P50454-1])
Modification sites at PhosphoSitePlus (P50454)
Glycosylation from GlyConnect SERPH_HUMAN (658)
Glycosylation from GlyGen (P50454) 6 sites, 28 N-linked glycans (2 sites), 1 O-linked glycan (4 sites)
- Protein Refseq
- XP_024304524.1
- Function
- Binds specifically to collagen.
Could be involved as a chaperone in the biosynthetic pathway of collagen.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SERPINH1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2872z | Mouse Anti-SERPINH1 Recombinant Antibody (clone 1B4) | WB, ICC, IF | Mouse IgG1, κ |
| MOB-0879CT | Recombinant Mouse anti-Human SERPINH1 Monoclonal antibody (EML235) | ICC, IF, IHC-P, WB | |
| MRO-1943-CN | Rabbit Anti-SERPINH1 Polyclonal Antibody (MRO-1943-CN) | WB, IF, IHC | Rabbit IgG |
| ZG-0559U | Rabbit Anti-SERPINH1 Recombinant Antibody (clone 10B2) | ELISA, IHC | Rabbit IgG |
| VS3-FY2870 | Mouse Anti-Serpinh1 Recombinant Antibody (VS3-FY2870) | WB, IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3228 | Hi-Affi™ Recombinant Rabbit Anti-SERPINH1 Monoclonal Antibody (DS3228AB) | WB, IHC-P, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6499 | Anti-SERPINH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6500 | Anti-Rat SERPINH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6501 | Anti-Mouse SERPINH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6502 | Anti-Hamster SERPINH1 Immunohistochemistry Kit | IHC |
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